A SIMPLE METHOD FOR THE PREPARATION OF THE IMMUNOGLOBULINS TO HYDROXYINDOLE O-METHYLTRANSFERASE

A simple procedure is described for the preparation of the monospecific immunoglobulins (Ig) to bovine pineal hydroxyindole O-methyltransferase (HIOMT) (EC 2.1.1.4). We observed that anti-serum to HIOMT was produced in rabbits which had received subcutaneous inoculations of the crude pineal extract. The monospecific Ig to HIOMT were isolated by an immunoadsorption technique in which the brain extract was coupled to the CNBr-activated Sepharose. The purified Ig gave a single precipitin line against the pineal extract on an Ouchterlony double diffusion plate and also by immunoelectrophoresis. All other extracts tested, cerebral cortex, cerebellum, thalamus, caudate nuclei, superior coliculus, liver, adrenal gland, kidney, retina and serum did not express precipitin lines to the purified Ig. When immunochemical titration was used, the pineal HIOMT was quantitatively precipitated by the monospecific Ig.     

木材腐朽菌培养特性的研究综述

论述了研究木材腐朽菌培养特性的意义,对自1889年以来有关木材腐朽菌培养特性的研究文献进行了回顾。通过综合分析以往国内外对木材腐朽菌培养特性研究的结果,得出了对木材腐朽菌培养特性研究的总体结论。     

A NEW TECHNIQUE FOR ASSESSING THE CONTACT TOXICITY OF MOLLUSCICIDES TO SLUGS

A technique is described to accurately assess the dermal contactdose-mortality response of molluscicides. The technique involvescarbon dioxide anaesthesia of laboratory reared Deroceras reticulatum(Müller) followed by application of test substances insolution to the dorsal surface. The LD₅₀ of the substances testedwere as follows: Sodium pentachlorophenate, 55.9 ng.mg^–1,Copper sulphate, 124.1 ng.mg^–1, Methiocarb, 314.6 ng.mg^–1and Potassium permanganate, 418.9 ng.mg^–1 body weight. (Received 18 August 1988; accepted 17 December 1988)     

一种纯化细菌有机磷水解酶的简便方法

Cibacron blue T_3GA与溴化氰活化的Sepharose 4B偶联后,产生一种能有效地分离有机磷水解酶的吸附剂。用0.15mol/L MgCl_2溶液从黄杆菌P3—2细胞抽提出的粗酶液通过柱层析分离,即可得到纯化8倍、酶活性回收率为269.4％的纯酶制品。该酶制品用凝胶电泳测是均一的。     

几种简便的木质素降解真菌定性筛选方法

简要介绍几种在前人基础上作了较大改进的木质素降解真菌的定性筛选方法,列出了每种方法的具体步骤并作了讨论。     

10种针阔叶树上常见的木材腐朽菌的培养特性*

描述了10种针阔叶树上常见的多孔菌的培养特性。它们是环纹革盖菌、蔷红拟层孔菌、盘拟层孔菌、斑褐孔菌、全缘孔菌、小刺猴头、黄白卧孔菌、烟色刺耳、变红栓菌和红斑干酪菌。