急性呼吸道感染儿童中WU多瘤病毒基因组序列特征分析

为了解从北京地区急性呼吸道感染儿童中发现的WU多瘤病毒的基因组编码特征,并对其进行基因序列多样性分析,应用针对基因组5'端非编码区、衣壳蛋白VP1、VP2编码基因以及LTAg编码基因的引物对,从已确证为WU病毒阳性的来自北京地区急性呼吸道感染儿童的编号为BJF5276的临床标本中经聚合酶链反应扩增得到预期的基因片段,直接测序后将序列拼接得到全基因组序列,进而推导其基因组编码特征;随后从其它21例已确证为WU多瘤病毒阳性的急性呼吸道感染儿童标本中扩增得到衣壳蛋白VP2编码区基因,进行基因序列测定以及基因序列多样性分析。得到了WU病毒BJF5276全基因组序列。序列分析结果显示WU病毒BJF5276基因组序列全长为5229bp,共有5个主要的CDS(Coding domain sequences),分别编码衣壳蛋白VP2、VP3、VP1,并以其互补序列为模板,编码STAg和LTAg;所得到的22例VP2蛋白编码区基因序列同源性比较结果显示病毒VP2基因编码区序列与GenBank中已有的64个序列之间同源性很高;Mega4.0NJ进化树(Neighbor-joiningtree)分析显示这22个VP2基因序列分属于不同的基因进化簇,其中20个序列属于进化簇I中的Ia,另外2个序列属于进化簇III,其中的一个序列在IIIb基因进化簇中,另外一个序列独立成簇,不属于现有的IIIa或IIIb,暂时将其命名为IIIc。本研究结果提示北京地区的WU病毒具有多瘤病毒科的基因组编码特性;序列非常保守,有分属于不同基因进化簇的WU病毒在北京地区流行,与文献报道的以Ib流行为主所不同的是北京地区的WU病毒以Ia为主,且有新的基因进化簇出现。     

中国小反刍兽疫病毒分离株China/Tib/07全基因组序列测定与分析

小反刍兽疫病毒属于副黏病毒科麻疹病毒属成员。本研究对我国首次分离的小反刍兽疫病毒株China/Ti-bet/07进行了全基因组序列测定及分子生物学特征分析。根据GenBank公布的小反刍兽疫病毒基因组序列设计引物通过RT-PCR扩增病毒基因组内部序列,通过3′和5′-RACE获得病毒基因组末端序列。序列测定与分析的结果表明,China/Tib/07株全长15948bp,预测编码6种结构蛋白和2种非结构蛋白,与已发表小反刍兽疫病毒基因组的长度和结构相似;在系统进化上与西南亚流行毒株有很高的同源性(91.6%～98.1%);与麻疹病毒属的其它成员相比,与牛瘟病毒的同源性最高(64.3%)。     

2010年云南省1株ECHO-9病毒全基因序列的遗传特性分析

为了解云南省手足口病患者标本中分离到的一株ECHO-9病毒基因组特征,对2010年云南省ECHO-9病毒分离株MSH-KM812-2010全基因组序列测序,并与GenBank中其它ECHO-9病毒株基因组序列比对和分析。MSH-KM812-2010基因组长为7 424bp,编码2 203个氨基酸,其结构基因区与其它ECHO-9病毒核苷酸和氨基酸的同源性高于其它型别肠道病毒;而在非结构区,与其它肠道病毒血清型的同源性高于ECHO-9病毒株。VP1系统进化分析显示ECHO-9病毒株可形成A、B和C三个进化分支,MSH-KM812-2010株以及其它中国分离株属于C簇。三个分支之间核苷酸序列的差异大于15.0%可将ECHO-9病毒分为三个基因型。通过重组检测软件3(RDP3)和基本局部比对搜索工具(BLAST)比对分析,发现在非结构区可能存在重组。本文首次对我国分离的ECHO-9病毒全基因组序列的测定和分析,对了解ECHO-9病毒遗传特性具有重要意义。     

GI型诺如病毒湖州株2008/Huzhou/N11进行全基因组序列测定与分析

为了对GI型诺如病毒湖州株2008/Huzhou/N11进行全基因组序列测定,了解其分子特征及基因类型。方法是根据GenBank上下载的GI型诺如病毒各基因型代表株保守序列设计特异性引物对2008/Huzhou/N11进行全基因组测序,并与GI型诺如病毒各基因型的原型株进行全序列的比对,使用MEGA 4.0软件绘制系统进化树。利用Simplot3.5.1软件进行相似性分析,对基因重组加以验证。结果表明,诺如病毒2008/Huzhou/N11基因组全长7 691bp,有3个开放阅读框(ORF)。ORF1长5 367bp(5~5 371nt),ORF2长1 623bp(5 355~6 977nt),ORF3长630bp(6 977~7 606nt)。RNA聚合酶区(4 320~5 091bp)系统进化分析显示2008/Huzhou/N11属于GI.2基因型,VP1区(5 355~6 977bp)和VP2区(6 977~7 606bp)的系统进化分析显示2008/Huzhou/N11属于GI.6基因型。进一步的Simplot分析提示2008/Huzhou/N11为GI.2/GI.6重组株,重组位点位于ORF1和ORF2重叠区的上游。本文确定了我国GI型诺如病毒湖州株2008/Huzhou/N11为GI.2/GI.6重组株,可为国内诺如病毒的遗传进化研究提供序列参考。     

牛血液中一株新型环状病毒的分离与全基因组序列分析

我们从广西壮族自治区哨兵动物牛上采集的血液样本中分离到一株病毒,暂将其命名为广西环状病毒(毒株号:V172/GX/2015)。病毒接种C6/36细胞后可产生明显的细胞病变,表现为细胞聚集、皱缩与脱落。高分辨率琼脂糖凝胶电泳显示,病毒基因组由10节段的双链RNA组成,在凝胶上呈现"3-4-3"的带型特征。通过全长cDNA扩增与高通量测序方法获取V172/GX/2015毒株的全基因组序列。病毒基因组大小为19 889bp,基因节段的大小在3 996bp(Seg-1)至829bp(Seg-10)之间,可编码VP1至VP7等7种结构蛋白以及NS1至NS3等3种非结构蛋白。对环状病毒属保守的VP1、VP3(T2)与VP7(T13)蛋白氨基酸序列分析与系统发育树分析显示,V172/GX/2015与蚊传播环状病毒Yunnan orbivirus、Peruvian horse sickness virus以及Mobuck virus具有较近的亲缘关系,氨基酸序列相似度在31.4%至69.9%之间;V172/GX/2015在系统发育树上形成了一个独立于其它环状病毒的进化分支。本研究首次报道了一种新型环状病毒在牛上的分离与全基因组序列,研究结果将进一步丰富我们对环状病毒属病毒的认知,为开展新型环状病毒的流行病学调查与致病性研究提供基础。     

家蚕传染性软化病病毒（桐乡株）VP1基因片段的克隆及序列分析

以首株在中国分离到的家蚕传染性软化病病毒（Bombyx mori infectious flacherie virus,BmIFV）BmIFV-CHN001基因组为模板,扩增了编码主要结构蛋白的VP1基因。克隆测序后得到VP1基因片段906 bp。该序列与已发表的日本毒株相比,核苷酸序列的相似性为99.3%,编码氨基酸的相似性为100%,证明该毒株与家蚕传染性软化病病毒日本株的同源性较高。把BmIFV-CHN001的VP1序列与同属的另外6个昆虫小RNA病毒的结构蛋白进行序列比对,构建系统发育树,对其进化关系进行了初步分析,结果显示这7种病毒具有相近的亲缘关系,而BmIFV-CHN001与蜜蜂囊雏病毒的亲缘关系最近。     

一株辛德毕斯病毒的基因组序列测定及分析

目的：对引进的一株辛德毕斯病毒的基因组序列进行测定,阐明其与已报道毒株序列的关系。方法：对辛德毕斯病毒基因组编码区进行分段RT-PCR扩增,对非编码区采用RACE法进行扩增,将扩增产物直接进行测序,应用DNAStar软件将测序结果拼接得到基因组序列,采用MEGA3.1软件对9株辛德毕斯病毒基因组序列进行系统进化发生树的构建。结果与结论：此株辛德毕斯病毒基因组共11663nt,编码3745个氨基酸残基,其中5＇端的2/3基因组编码4种非结构蛋白NSp1、NSp2、NSp3和NSp4,3＇端的1/3基因组编码5种结构蛋白E1、E2、E3、6K和C;结构基因和非结构基因之间有48nt的连接区为非翻译区;病毒基因组5＇末端和3＇末端分别有59、318nt的非编码区;序列同源性分析结果表明,此株病毒与S.A.AR86株的同源性最高,两者核苷酸序列的同源性为99.7%,氨基酸序列的同源性为99.6%,而与本室保存的另一辛德毕斯病毒MEI株的遗传进化关系稍远,系统进化发生树处于不同分支上。     

福建一例HAstV-5型星状病毒的全基因组测序与重组分析

为了探究一例福建省检出的HAstV-5型星状病毒2013/Fuzhou/85毒株基因组分子结构特点,本研究采用PCR分段扩增、测序、拼接的方法,获得2013/Fuzhou/85毒株基因组序列全长6 803bp:5’端和3’端均有85bp非编码区;中间3个开放阅读框:ORF1a长2 802bp(86～2 887nt),编码非结构蛋白丝氨酸蛋白酶;ORF1b长1 548bp(2 827～4 374nt),编码非结构蛋白RNA聚合酶;ORF2长2 352bp(4 367～6 718nt),编码结构蛋白衣壳蛋白前体。目前,GenBank中仅有两株HAstV-5型星状病毒全基因组序列:中国辽宁毒株(JQ403108)和巴西哥亚尼亚毒株(DQ028633),2013/Fuzhou/85毒株和中国辽宁毒株核苷酸相似度最高,达94.4%。对该HAstV-5型星状病毒3个开放阅读框分别构建系统进化树,发现ORF1a与HAstV-1(JF327666)相似度最高,ORF1b和ORF2与HAstV-5(JQ403108)相似度最高,提示其有可能存在重组,用Simplot软件进行重组分析,重组位点位于2 741bp,在ORF1a和ORF1b重叠区的上游。本研究中对2013/Fuzhou/85毒株的全基因组测序和重组分析,可以为星状病毒的重组和遗传进化规律研究提供参考。     

新成人腹泻轮状病毒J19株的全基因组克隆和VP4、VP6和VP7基因序列分析

为了进一步了解新成人腹泻轮状病毒J19株的基因和蛋白特征,利用一种改良的非依赖核酸序列的单引物扩增方法扩增J19株的11个基因,克隆到pMD18-T载体中并进行测序。在此基础上,将主要抗原蛋白VP4、VP6和VP7的蛋白序列与其它轮状病毒的相关蛋白序列进行比较分析并对VP6蛋白序列做遗传进化分析。结果获得J19株11个基因的全长基因序列。基因序列分析表明J19株的第3、6和第9基因分别长2 512bp、1 287bp和820bp,它们分别预测编码抗原蛋白VP4(823aa)、VP6(396aa)和VP7(258aa)。组成J19株的VP4、VP6和VP7蛋白序列对B组轮状病毒的CAL株、IDIR株以及ADRV株的相关蛋白序列的一致性分别是27.6%、38.5%和22.3%。对分组抗原蛋白VP6的遗传进化分析表明,J19株在进化树上的位置靠近外群蛋白分支以及A、B和C组轮状病毒分支的根部,而且它比较偏向于B组轮状病毒的分支。J19株的VP4、VP6和VP7蛋白序列与其它轮状病毒的相应蛋白序列存在显著差异。VP6蛋白序列的遗传进化分析表明J19株可能是一个新组轮状病毒的代表性毒株;同时,它也可能是一个与B组轮状病毒的起源和进化密切相关的毒株之一。关于新成人腹泻轮状病毒J19株11个基因的克隆及VP4、VP6和VP7基因的序列分析,这是第一次报道。     

云南省墨江县库蠓中一株西藏环状病毒的分离及全基因组序列分析

西藏环状病毒（Tibet orbivirus,TIBOV）于2009年首次从中国西藏自治区采集的圆斑按蚊中分离出,目前在中国的云南省、广东省、湖南省和临国日本均有分离报道。2017年我们从云南省墨江县采集的库蠓样品中分离到一株病毒（MJC1-7）,接种BHK-21和C6/36细胞后均可产生聚集、皱缩和脱落等明显细胞病变。1%的琼脂糖凝胶电泳显示,病毒基因组为十节段的双链RNA,呈现“3-3-3-1”的带型。通过病毒全长cDNA扩增和测序获取MJC1-7毒株的全基因组核苷酸序列,病毒基因组全长为19 260 bp（包括编码区18 495 bp和非编码区735 bp）,由Seg-1(3 950 bp)至Seg-10(832 bp)的10个基因节段构成,可编码6 165个氨基酸残基。对环状病毒保守的VP1(Pol)、VP3(T2)和VP7(T13)蛋白氨基酸序列及系统发育分析显示,MJC1-7毒株与TIBOV亲缘关系最近,氨基酸序列相似度为95.4%～99.7%。对决定环状病毒血清型VP2(OC1)蛋白氨基酸序列与系统发育分析显示,MJC1-7与云南（SX-2017a、DH13C120和YN...     

Diversity of Coding Sequences and Gene Structures of the Antifungal Peptide Mytimycin (MytM) from the Mediterranean Mussel, <Emphasis Type="Italic">Mytilus galloprovincialis</Emphasis>

Knowledge on antifungal biomolecules is limited compared to antibacterial peptides. A strictly antifungal peptide from the blue mussel, Mytilus edulis named mytimycin (MytM) was reported in 1996 as partial NH₂ 33 amino acid sequence. Using back-translations of the previous sequence, MytM-related nucleotide sequences were identified from a normalized Mytilus galloprovincialis expressed sequence tag library. Primers designed from a consensus sequence have been used to obtain a fragment of 560 nucleotides, including the complete coding sequence of 456 nucleotides. Precursor is constituted by a signal peptide of 23 amino acids, followed by MytM of 54 amino acids (6.2–6.3 kDa, 12 cysteines) and C-terminal extension of 75 amino acids. Only two major amino acid precursor sequences emerged, one shared by M. galloprovincialis from Venice and Vigo, the other belonging to M. galloprovincialis from Palavas, with nine amino acid differences between the two MytM. Predicted disulfide bonds suggested the presence of two constrained domains joined by amino acidic NIFG track. Intriguing was the presence of conserved canonical EF hand-motif located in the C-terminus extension of the precursor. The MytM gene was found interrupted by two introns. Intron 2 existed in two forms, a long (1,112 nucleotides) and a short (716 nucleotides) one resulting from the removal of the central part of the long one. Both the short (GenBank FJ804479) and the long (GenBank FJ804478) genes are simultaneously present in the mussel genome.     

PCR detection and sequence analysis of duck circovirus in sick Muscovy ducks

The duck circovirus (DuCV) infection in sick ducks from Fujian Province was investigated. The liver samples of 43 sick Muscovy ducks with infectious serositis were collected from 12 duck farms in Fujian Province.Based on the published sequences of DuCV, two primers were designed for the detection of DuCV and four pairs of primers were designed to amplify four overlapping fragments that cover the complete genome of DuCV. The specific PCR products were amplified from positive samples. The fragments were then cloned into pMD18-T vector and sequenced, and the full length genomic sequence of the FJ0601 isolate of DuCV was obtained. PCR analysis showed that the proportion of ducks which were positive for circovirus was 79% and 10 out of the 12 farms were positive. Sequence analysis showed that the complete genome of DuCV-FJ0601 was 1988 bp and possessed features common to the family Circoviridae which included a stem-loop structure and the Rep protein motifs. Homology analysis showed that FJ0601 isolate of DuCV had 97.3%～97.5% nucleotide sequence identity to all the four Taiwan isolates (TC1/2002, TC2/2002, TC3/2002, TC4/2002), 82.9% identity to the America (33753-52) isolate and 82.3% identity to the Germany isolate. Phylogenetic analysis with Clustal W, however,showed that FJ0601 isolate of DuCV was on a common branch with Taiwan isolates, and Germany and America isolates belonged to the other branch.     

The Complete Genome Phylogeny of Geographically Distinct Dengue Virus Serotype 2 Isolates (1944-2013) Supports Further Groupings within the Cosmopolitan Genotype

Dengue virus serotype 2 (DENV-2) isolates have been implicated in deadly outbreaks of dengue fever (DF) and dengue hemorrhagic fever (DHF) in several regions of the world. Phylogenetic analysis of DENV-2 isolates collected from particular countries has been performed using partial or individual genes but only a few studies have examined complete whole-genome sequences collected worldwide. Herein, 50 complete genome sequences of DENV-2 isolates, reported over the past 70 years from 19 different countries, were downloaded from GenBank. Phylogenetic analysis was conducted and evolutionary distances of the 50 DENV-2 isolates were determined using maximum likelihood (ML) trees or Bayesian phylogenetic analysis created from complete genome nucleotide (nt) and amino acid (aa) sequences or individual gene sequences. The results showed that all DENV-2 isolates fell into seven main groups containing five previously defined genotypes. A Cosmopolitan genotype showed further division into three groups (C-I, C-II, and C-III) with the C-I group containing two subgroups (C-IA and C-IB). Comparison of the aa sequences showed specific mutations among the various groups of DENV-2 isolates. A maximum number of aa mutations was observed in the NS5 gene, followed by the NS2A, NS3 and NS1 genes, while the smallest number of aa substitutions was recorded in the capsid gene, followed by the PrM/M, NS4A, and NS4B genes. Maximum evolutionary distances were found in the NS2A gene, followed by the NS4A and NS4B genes. Based on these results, we propose that genotyping of DENV-2 isolates in future studies should be performed on entire genome sequences in order to gain a complete understanding of the evolution of various isolates reported from different geographical locations around the world.     

Isolation of novel mycobacteria contaminating an aquarium fish tank in a Japanese university hospital

Aims: To better understand nontuberculous mycobacteria (NTM) contamination in a hospital setting, six freshwater fish gut homogenates and water in an aquarium fish tank placed on the reception counter of a nursing station were cultured for mycobacteria. Methods and Results: By direct sequencing of 16s rRNA, rpoB and hsp65, scotochromogenic and nonchromogenic Mycobacterium szulgai isolates containing hsp65 type II (GenBank accession nos. FJ384762 and FJ384764 , respectively), Mycobacterium gordonae isolates containing rpoB clusters B and E (GenBank accession no. FJ384766 ), and Mycobacterium kansasii isolates containing hsp65 type VI were collected from the gut homogenates and water from the fish tank. However, no isolates were obtained from the tap water used to refill the fish tank. A randomly amplified polymorphic DNA (RAPD) analysis using a 10‐mer primer (5′‐TGGTCGCGGC) showed that some NTM from the fish tank water were identical to those obtained from the gut homogenates. Conclusions: Fish and water in the tank were contaminated by the novel NTM. Significance and Impact of the Study: These findings could help to elucidate infection routes and contamination sources of novel NTM from water sources.     

中国6株狂犬病病毒街毒株全基因组测序与分析

实验研究中对分离于中国的6株狂犬病病毒街毒株进行了全基因组测序,对基因组的5个结构基因(N、P、M、G和L)的核苷酸和推断的氨基酸序列以及非编码区序列进行了分析与比较,并与来自GenBank的40株毒株从全基因组水平进行了分子进化分析。所测6株中国狂犬病病毒街毒株的全基因组核苷酸序列长度介于11 907 nt(CQ92)和11 924 nt(SH06和gg4)之间,基因组结构相同,用全基因组和不同的结构基因构建的进化树拓扑结构相似,基因组3′和5′末端高度保守而且末端11个核苷酸互补配对,5个结构基因的保守性依次是NLMGP,核苷酸同源性的最小值依次分别是81.9%、81.7%、80.7%、78.3%和76.7%。     

A gain-of-function screen in zebrafish identifies a guanylate cyclase with a role in neuronal degeneration

Manipulation of gene expression is one of the most informative ways to study gene function. Genetic screens have been an informative method to identify genes involved in developmental processes. In the zebrafish, loss-of-function screens have been the primary approach for these studies. We sought to complement loss-of-function screens using an unbiased approach to overexpress genes with a Gal4-UAS based system, similar to the gain-of-function screens in Drosophila. Using MMLV as a mutagenic vector, a cassette containing a UAS promoter was readily inserted in the genome, often at the 5′ end of genes, allowing Gal4-dependent overexpression. We confirmed that genes downstream of the viral insertions were overexpressed in a Gal4-VP16 dependent manner. We further demonstrate that misexpression of one such downstream gene gucy2F, a membrane-bound guanylate cyclase, throughout the nervous system results in multiple defects including a loss of forebrain neurons. This suggests proper control of cGMP production is important in neuronal survival. From this study, we propose that this gain-of-function approach can be applied to large-scale genetic screens in a vertebrate model organism and may reveal previously unknown gene function. Sequence data from this article have been deposited with the EMBL/GenBank Data Libraries under accession nos. FJ151012, FJ151013, and FJ151014.     

Complete Genomic Sequence and Comparative Analysis of the Genome Segments of Sweet Potato Chlorotic Stunt Virus in China

Background

Sweet potato chlorotic stunt virus (family Closteroviridae, genus Crinivirus) features a large bipartite, single-stranded, positive-sense RNA genome. To date, only three complete genomic sequences of SPCSV can be accessed through GenBank. SPCSV was first detected from China in 2011, only partial genomic sequences have been determined in the country. No report on the complete genomic sequence and genome structure of Chinese SPCSV isolates or the genetic relation between isolates from China and other countries is available.

Methodology/Principal Findings

The complete genomic sequences of five isolates from different areas in China were characterized. This study is the first to report the complete genome sequences of SPCSV from whitefly vectors. Genome structure analysis showed that isolates of WA and EA strains from China have the same coding protein as isolates Can181-9 and m2-47, respectively. Twenty cp genes and four RNA1 partial segments were sequenced and analyzed, and the nucleotide identities of complete genomic, cp, and RNA1 partial sequences were determined. Results indicated high conservation among strains and significant differences between WA and EA strains. Genetic analysis demonstrated that, except for isolates from Guangdong Province, SPCSVs from other areas belong to the WA strain. Genome organization analysis showed that the isolates in this study lack the p22 gene.

Conclusions/Significance

We presented the complete genome sequences of SPCSV in China. Comparison of nucleotide identities and genome structures between these isolates and previously reported isolates showed slight differences. The nucleotide identities of different SPCSV isolates showed high conservation among strains and significant differences between strains. All nine isolates in this study lacked p22 gene. WA strains were more extensively distributed than EA strains in China. These data provide important insights into the molecular variation and genomic structure of SPCSV in China as well as genetic relationships among isolates from China and other countries.     

生姜根际茎腐病拮抗菌的筛选及鉴定

从生姜根际土样分离获得的86个细菌分离物中,通过离体拮抗试验,筛选出2株对生姜茎腐病有良好拮抗效果的细菌,编号分别为JF24和JF3。通过对它们进行形态学观察、生理生化测定以及16S rRNA序列分析,初步确定,JF24为荧光假单胞菌(Pseudomonas fluorescens),JF3为粪产碱菌(Alcaligenes faecalis)。JF24和JF3的16S rRNA序列的GenBank登录号分别为FJ999730和FJ999731。     

Tandem organization of StarkB element (22.8 kb) in the maize B chromosome

A very large repetitive element (StarkB, 22.8 kb) is present in the maize B chromosome, presumably not organized as tandem arrays. Results of the current study are contrary to this notion. Out of eighteen StarkB-carrying sequences, nine were the expected internal fragment of StarkB, and nine others were fragments spanning two StarkB elements. One of the two StarkB components, GrandeB, was flanked in all clones with identical target sequences, as opposed to other Grandes that are associated with different target sequences. Also observed was a prominent Southern signal associated with a fragment representing the junction of two adjacent StarkB units. A clone possessing a structure inverse to that of the second component of StarkB is proposed to be the initial element into which a GrandeB inserted to derive StarkB. Most, if not all, isolated StarkB arrays were not the original form, being disrupted by the invasion of various mobile elements intertwined with various stages of amplification. Electronic supplementary material  The online version of this article (doi:) contains supplementary material, which is available to authorized users. Nucleotide sequence data reported are available in the GenBank database under the accession numbers EF468500 to EF468511, EU528676, and FJ386410 to FJ386429.     

Complete Genome of a Porcine Calicivirus Strain in Anhui Province,China, Is Significantly Shorter than That of the Other Chinese Strain

Caliciviruses that cause diarrhea have been reported in both industrial and developing countries, including China, in recent years. Here, we report the complete genome of a porcine calicivirus strain, Ah-1, which is prevalent in swine groups in Anhui Province. This viral genome is 7,342 nucleotides (nt) long, excluding the poly(A) of the 3′ end, which is 202 nt shorter in the 3′ untranslated region (UTR) than that of the other Chinese porcine calicivirus strain (Ch-sw-sav1; GenBank accession number {"type":"entrez-nucleotide","attrs":{"text":"FJ387164","term_id":"227976393","term_text":"FJ387164"}}FJ387164), previously isolated in the Shanghai area, China, though they shared 98.8% sequence identity over the whole genome excluding the 202-nt-shorter region.