内皮素受体反义寡聚核苷酸对内皮素受体基因表达及血管平滑肌细胞增殖的影响

报道了内皮素Ａ型受体反义寡聚核苷酸（ＯＤＮｓ）对大鼠血管平滑肌细胞（ＶＳＭＣ）增殖及内皮素受体基因表达的影响．~３Ｈ－ＴｄＲ参入结果显示,内皮素Ａ型受体反义ＯＤＮｓ处理细胞可显著抑制内皮素诱导的ＶＳＭＣ的ＤＮＡ合成,反转录－ＰＣＲ及受体结合实验结果表明,ＯＤＮｓ的上述作用与降低ＶＳＭＣ内皮素Ａ型受体基因表达活性有关．     

佛波酯能改变细胞表面糖蛋白N－糖链的结构

利用标记Ｎ－糖链的凝集素亲和层析法研究了佛波醇肉桂酸乙酸酯（ＰＭＡ）对人肝癌细胞ＳＭＭＣ－７７２１表面糖蛋白上Ｎ－糖链结构的影响,发现１００ｎｍｏｌ／Ｌ的ＰＭＡ处理５天后,可使细胞表面Ｎ－糖链中高甘露糖型和杂合型以及四天线、Ｃ２Ｃ２,６三天线复杂型的比例增高,而二天线复杂型降低。此结果与我们曾报道的视黄酸（ＲＡ）和双丁配环磷酸腺苷（ｄｂ－ｃＡＭＰ）对该细胞表面Ｎ－糖链的影响相反。因ＲＡ和ｄｂ－ｃＡＭＰ是ＳＭＭＣ－７７２１细胞的分化诱导剂,可抑制细胞生长;而ＰＭＡ是该细胞的增殖促进剂,故细胞表面Ｎ－糖链的变化与细胞的分化和增殖密切相关。     

中国跗线螨科三新纪录神(蜱螨亚纲:真螨目)

中国跗线螨科三新纪录神（蜱螨亚纲：真螨目）ＴＨＲＥＥＮＥＷＬＹＲＥＣＯＲＤＥＤＳＰＥＣＩＥＳＯＦＴＡＲＳＯＮＥＭＩＤＡＥＦＲＯＭＣＨＩＮＡ（ＡＣＡＲＩ：ＡＣＡＲＩＦＯＲＭＥＳ）￥ＬＩＮＪｉａｎ－ｚｈｅｎ;ＺＨＡＮＧＹａｎ－ｘｕａｎ（Ｉｎｓｔｉｔｕｔｅ...     

人肝纤维化中肌纤维母细胞的组织原位研究

本文以α－平滑肌肌动蛋白（α－ＳＭＡ）作为肌纤维母细胞（ＭＦｂ）的标志,对７３例人肝组织作免疫组化研究,观察α－ＳＭＡ阳性细胞与Ⅰ、Ⅲ型胶原以及炎症细胞的关系。发现在各种慢性肝病的肝组织中α－ＳＭＡ、Ⅰ、Ⅲ型胶原阳性率均明显高于对照组;尤其在慢性活动性病变时,α－ＳＭＡ阳性细胞数与Ⅲ型胶原量均多,而在慢性非活动性病例中Ⅰ型胶原量较多;且各组显示α－ＳＭＡ阳性细胞量的曲线与Ⅲ型胶原基本一致。α－ＳＭＡ阳性细胞的增生及分布与炎症细胞的浸润也密切相关。因此,可籍ＭＦｂ（α－ＳＭＡ阳性）的定位检测来判断人肝纤维化的进展情况。     

关于平列型气孔定义的讨论

关于平列型气孔定义的讨论汪乐原（湖北药检高等专科学校,武汉４３００６４）ＡＤＩＳＣＵＳＳＩＯＮＯＮＴＨＥＤＥＦＩＮＩＴＩＯＮＳＯＦＰＡＲＡＣＹＴＩＣＳＴＡＭＡＴＡＷａｎｇＬｅ－ｙｕａｎ（ＨｕｂｅｉＤｒｕｇＣｏｎｔｒｏｌａｎｄＭｅｄｉｃａｌＥｘａｍｉｒ...     

一氧化氮抑制内皮素促血管平滑肌细胞增殖作用的信号转导途径

培养的家兔胸主动脉血管平滑肌细胞（ＶＳＭＣ）分别以内皮素（ＥＴ－１）、一氧化氮（ＮＯ）前体Ｌ－Ａｒｇ和ＮＯ供体ＳＩＮ－１刺激,或用ＥＴ－１＋Ｌ－Ａｒｇ、ＥＴ－１＋ＳＩＮ－１联合刺激,测ＶＳＭＣ＾３Ｈ－ＴｄＲ掺入、丝裂素活化蛋白激酶（ＭＡＰＫ）活性及蛋白激酶Ｃ（ＰＫＣ）活性的改变,以研究ＮＯ抑制ＥＴ－１促ＶＳＭＣ增殖作用的信号转导途径。结果表明：（１）ＥＴ－１ １０＾－８ｍｏｌ／Ｌ单独刺激,＾３Ｈ－     

强啡肽对脊髓原生型与诱生型一氧化氮合成酶活性、分布和基因表达的影响及机制

蛛网膜下腔注射（ｉ．ｔ．）强啡肽Ａ１－１７（Ｄｙｎ）引起剂量依赖性后肢和尾部瘫痪及甩尾甩足抑制。脊髓背角（侧）ＮＭＤＡ受体和ＮＯＳ／ＮＯ功能活性下降可能与Ｄｙｎ镇痛作用有关,脊髓腹角（侧）ＮＭＤＡ受体－Ｃａ２＋－ＮＯＳ／ＮＯ通路过度激活及ｃ－ｆｏｓ高表达可能与Ｄｙｎ致脊髓损伤（ＳＣＩ）作用有关。在Ｄｙｎ致ＳＣＩ机制中,过量ＮＯ（细胞水平）具有神经毒性作用,脑源性ＮＯＳ主要在早期,诱生型ＮＯＳ主要在后期起作用,而内皮细胞源性ＮＯＳ和适量ＮＯ（血管水平）可能具有保护作用。在原代培养脊髓神经元中,高浓度Ｄｙｎ可通过ＮＭＤＡ受体和κ受体直接引起细胞内Ｃａ２＋超负荷,低浓度Ｄｙｎ只通过κ受体抑制高钾刺激性Ｃａ２＋内流     

新麦草的组织培养及染色体分析

新麦草的组织培养及染色体分析孙震晓,夏光敏,陈惠民（山东大学生物系．济南２５０１００）ＴＩＳＳＵＥＣＵＬＴＵＲＥＡＮＤＣＨＲＯＭＯＭＥＡＮＡＬＹＳＩＳＯＦＰｓＡＴＨＹＲＯＳＡＣＨＹＳＪＵＮＣＥＡ（ＦＩＳＣＨ．）ＮＥＶＳＫＩｓｕｎＺｈｅｎ－ｘｉａｏ;ｘ...     

池杉─水稻系统的生态效应（Ⅱ）系统的生态环境效应

池杉┐水稻系统的生态效应（Ⅱ）系统的生态环境效应黄兆祥郑珍贵朱笃（南昌大学生物科学工程系,南昌３３００４７）ＥＣＯＬＯＧＩＣＡＬＥＦＦＥＣＴＯＦＴＡＸＯＤＩＵＭＡＳＣＥＮＤＥＮＳ－ＯＲＹＺＡＳＡＴＩＶＡＥＣＯＳＹＳＴＥＭ（Ⅱ）ＥＣＯＬＯＧＩＣＡＬ－...     

延髓腹外侧区微量注射L－NNA和SNP对大鼠血压、心率和肾交感神经活动的影响

在麻醉大鼠观察了向延髓腹外侧区微量注射ＮＯ合成酶抑制剂Ｎ－硝基左旋精氨酸（ＬＮＮＡ）和硝普钢（ＳＮＰ）对血压、心率和肾交感神经活动的影响,旨在探讨中枢左旋精氨酸－ＮＯ通路在动脉血压调节中的作用及其机制。实验结果如下：（１）向延髓腹外侧头端区（ＲＶＬＭ）注射Ｌ－ＮＮＡ后,平均动脉压（ＭＡＰ）升高,肾交感神经活动（ＲＳＮＡ）增强;心率（ＨＲ）减慢,但无统计学意义。ＭＡＰ和ＲＳＮＡ的变化持续３０ｍｉｎ以上;此效应可被预先静注左旋精氨酸所逆转。（２）向ＲＶＬＭ微量注射ＳＮＰ,ＭＡＰ降低,ＲＳＮＡ减弱;但ＨＲ的变化无统计学意义。（３）向延髓腹外侧尾端区（ＣＶＬＭ）注射Ｌ－ＮＮＡ,ＭＡＰ降低,ＨＲ减慢,ＲＳＮＡ减弱。（４）向ＣＶＬＭ微量注射ＳＮＰ,ＭＡＰ升高,ＲＳＮＡ增强,而心率无明显变化。以上结果表明,中枢左旋精氨酸－ＮＯ通路对延髓腹外侧部的神经元活动有调变作用。     

Recent advances in the study of Kaposi's sarcoma-associated herpesvirus replication and pathogenesis

It has now been over twenty years since a novel herpesviral genome was identified in Kaposi's sarcoma biopsies. Since then, the cumulative research effort by molecular biologists, virologists, clinicians, and epidemiologists alike has led to the extensive characterization of this tumor virus, Kaposi's sarcoma-associated herpesvirus(KSHV; also known as human herpesvirus 8(HHV-8)), and its associated diseases. Here we review the current knowledge of KSHV biology and pathogenesis, with a particular emphasis on new and exciting advances in the field of epigenetics. We also discuss the development and practicality of various cell culture and animal model systems to study KSHV replication and pathogenesis.     

The structure, reproduction and taxonomy of Vidalia and Osmundaria (Rhodophyta, Rhodomelaceae)

Comprises species occurring mostly in subtidal habitats in tropical, subtropical and warm-temperate areas of the world. An analysis of the type species, V. spiralis (Sonder) Lamouroux ex J. Agardh, a species from Australia, establishes basic characters for distinguishing species in the genus. These characters are (1) branching patterns of thalli, (2) flat blades that may be spiralled on their axis, (3) width of the blade, (4) primary or secondary derivation of sterile and fertile branchlets and (5) position of sterile and fertile branchlets on the thalli. Application of the latter two characters provides an important basic method for separation of species into three major groups. Osmundaria , a genus known only in southern Australia, was studied in relation to Vidalia , and its separation from the Vidalia assemblage is not accepted. Species of Vidalia therefore are transferred to the older genus name, Osmundaria. Two new species, Osmundaria papenfussii and Osmundaria oliveae are described from Natal. Confusion in the usage of the epithet, Vidalia fimbriala Brown ex Turner has been clarified, and Vidalia gregaria Falkenberg, described as an epiphyte on Osmundaria pro/ifera Lamouroux, is revealed to be young branches of the host, Osmundaria prolifera.     

New or rare chromosome counts in Artemisia L. (Asteraceae, Anthemideae) and related genera from Kazakhstan

Fifteen chromosome counts of six Artemisia taxa and one species of each of the genera Brachanthemum, Hippolytia, Kaschgaria, Lepidolopsis and Turaniphytum are reported from Kazakhstan. Three of them are new reports, two are not consistent with previous counts and the remainder are confirmations of very scarce (one to four) earlier records. All the populations studied have the same basic chromosome number, x = 9, with ploidy levels ranging from 2x to 6x. Some correlations between ploidy level, morphological characters and distribution are noted.     

肝癌中HBV和HCV基因和抗原的分布及意义

采用原位分子杂交方法检测HCV RNA及HBV X基因;采用免疫组织化学方法研究HCV核心抗原,非结构区C33c抗原及HBxAg在肝细胞肝癌中的定位及分布.结果表明(1)HCV RNA、HBV X基因在肝细胞肝癌组织检出率分别为40%(55/136)和82%(112/136).HCV RNA定位于癌细胞的胞浆内,阳性细胞呈散在、灶状及弥漫分布三种形式;HBV X基因在肝癌细胞中的分布呈胞浆型、核型及核浆型,阳性细胞也呈上述三种分布形式;(2)HCV C33c抗原、核心抗原在肝细胞肝癌中的阳性率为81%(133/164)及86%(141/164).C33c抗原定位于癌细胞及肝细胞的胞浆内;核心抗原既定位于癌细胞核中,又可定位于胞浆中.C33c抗原阳性细胞以灶状分布为主;而核心抗原阳性细     

Selection with two alleles and complete dominance

For a plant selection model with frequency-independent viabilities, fertilities and selfing rates, it is shown that apart from global fixation, for certain parameter combinations a protected polymorphism and facultative fixation (either allele may become fixed according to initial frequencies) may both occur. Facultative fixation requires different selling rates for the dominant and recessive type. Protection of the polymorphism requires resource allocation for male and female function. In this connection the problem of purely genetically caused population extinction is discussed.
For general frequency dependence and regular segregation, the chances for establishment of a completely recessive gene are compared to those of a completely dominant gene. It is proven that the process of establishment of the recessive gene, despite a fitness advantage, may be considerably endangered by drift effects if random mating prevails. The recessive gene may reach the same effectivity in establishment as a dominant gene, only if the recessive homozygote mates exclusively with its own type during the period of establishment.