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草莓试管苗分化培养基优化的研究 总被引:6,自引:0,他引:6
本研究采用三种培养基(A3、F、MS)进行草莓分化对比试验.结果表明我们研制的A3基对草莓试管苗分化有明显促进作用.连续培养11周,A3的草莓苗总分化率是F基的1.25倍,是MS基的1.51倍。而且,A3基成本低于其它两基。A3、F和MS无机盐含量分虽为:1782.58mg/L、2868.33mg/L和4633.33mg/L。无机盐种类的合理配比及其低浓度有利于草莓试管的分化培养。 相似文献
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用正交试验法筛选樟芝菌适宜发酵培养基 总被引:4,自引:0,他引:4
用正交试验法对樟芝菌 (AntrodiacamphorataZang&Su)的发酵培养基进行了研究。选取马铃薯淀粉、葡萄糖、麦芽糖、酵母膏、蛋白胨、麸皮、KH2 PO4 、MgSO4 ·7H2 O等作为试验因素 ,采用L2 7(31. 3)正交表设计五因素、三水平的正交试验 ,对所选各因素的添加量分别作了试验 ,对菌丝干重和发酵液多糖含量结果作了方差分析 ,确定了合适的发酵培养基。试验结果表明 :樟芝菌的深层发酵适宜培养基是 :马铃薯淀粉 1 .0 0 % ,葡萄糖2. 0 0 % ,酵母膏 0 . 10 % ,蛋白胨 0 . 10 % ,麸皮 1 .0 0 % (或 0. 5 0 % ) ,KH2 PO4 0 . 10 % ,MgSO4 ·7H2 O 0 . 0 5 %。 相似文献
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应用模式识别法优化发酵培养基,以培养基组成构筑模式空间,线性降维揭示可视优化区,再以多因素轮换优化实现由低维到高维的回复,得到最佳培养基组成。 相似文献
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目的 探讨施万细胞条件培养基对大鼠骨髓间质细胞的诱导分化作用。方法 从大鼠骨髓中分离培养间质细胞并传至第 6代 ,诱导前 2 4h加 1μg·L-1碱性成纤维生长因子 (bFGF)入培养液中以促分裂 ,再以施万细胞条件培养基作诱导剂 ,观察细胞形态的变化 ,并采用免疫组织化学法对诱导后一周的细胞进行Map 2及NSE、GFAP表达的检测。结果 诱导后 48小时间质细胞在形态上表现为神经元样 ,神经元样细胞呈Map 2及NSE阳性 ,而GFAP显阴性。结论 施万细胞的上清液能诱导骨髓间质细胞分化为神经元样细胞。 相似文献
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化学法研究长春花细胞胞内产物释放行为 总被引:3,自引:0,他引:3
对不同生理状态下细胞对外界刺激的反应情况进行了比较;研究了EDTA、氮酮、甲壳素做为长春花细胞渗透剂所使用的条件;探讨了它们影响胞内产物释放的短期效应、长期效应及长时间处理所造成的叠加效应。 相似文献
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应用正交设计方法优化香蕉外植体直接出芽的条件 总被引:7,自引:0,他引:7
利用正交设计方法(L27(3^13)]研究了培养基中的植物生长调节物BAP,NAA和IBA,以及不同外植体对香蕉(Musa acuminata Colla.cv.Williams) 直接出芽频率的影响,外植体的不同是影响香蕉直接出芽频率的最主要因素,NAA和IBA对实验结果的影响相同,均弱于BAP,实验结果的统计分析表明,诱导香蕉外植体直接出芽的优选培养基为不添加BAP,NAA和IBA的MS基本培养基,最佳外植体为芽的顶端分生组织部分。 相似文献
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Héla Mezghanni Saoussen Ben Khedher Slim Tounsi 《Preparative biochemistry & biotechnology》2013,43(3):267-278
In order to overproduce biofungicides agents by Bacillus amyloliquefaciens BLB371, a suitable culture medium was optimized using response surface methodology. Plackett–Burman design and central composite design were employed for experimental design and analysis of the results. Peptone, sucrose, and yeast extract were found to significantly influence antifungal activity production and their optimal concentrations were, respectively, 20 g/L, 25 g/L, and 4.5 g/L. The corresponding biofungicide production was 250 AU/mL, corresponding to 56% improvement in antifungal components production over a previously used medium (160 AU/mL). Moreover, our results indicated that a deficiency of the minerals CuSO4, FeCl3 · 6H2O, Na2MoO4, KI, ZnSO4 · 7H2O, H3BO3, and C6H8O7 in the optimized culture medium was not crucial for biofungicides production by Bacillus amyloliquefaciens BLB371, which is interesting from a practical point of view, particularly for low-cost production and use of the biofungicide for the control of agricultural fungal pests. 相似文献
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本文通过超临界CO2提取芝麻油的均匀设计实验和微波和超声波诱导提取芝麻油的正交实验比较,考察影响提取的主要因素,寻求最佳萃取工艺。超临界CO2萃取最佳工艺条件为:压力32MPa,温度60℃,CO2流量31kg/h,萃取时问80min,得率46.39%;微波萃取最佳工艺条件为:溶剂为丙酮,物料与溶剂比例1:7,辐射时间7min,辐射功率810W,得率23.01%。超声波萃取最佳工艺条件为:物料与溶剂比例1:7,溶剂为石油醚,浸泡时间30h,得率23.99%。结果表明超临界CO2萃取芝麻油品质最好,而且萃取也最高,质量最稳定。 相似文献
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按照多因素多水平的正交实验结果,制备猪血浆的最佳条件是猪血与8%柠檬酸三钠溶液体积比19:1,离心时间10分钟,转速1200转/分。用此条件制备的猪血浆与羊血浆进行对比试验,证明猪血浆可以用于肝素钠抗凝血效价测定。 相似文献
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Bacillus sp. are specific producers of peptidase amongst bacteria and peptidase enzymes and are of significant ones due to their multifarious applications. Advances in industrial biotechnology offer potential opportunities for economic utilization of agro-industrial by-products for many biochemical reactions. Due to their rich organic nature, they can serve as an ideal substrate for the production of different value added products like peptidases. In the present work, an attempt was made to optimize different variables by Taguchi methodology for the production of peptidase using agro-industrial by-products hydrolyzed by a Bacillus cereus strain, resulting in brewer's spent grain (BSG) being the optimal organic substrate. Subsequently, operative variables for the BSG were investigated using Taguchi methodology in order to maximize the enzyme production. Additionally, the main medium components were optimized using a mixture design. Finally, the production of peptidase by B. cereus was investigated; also the possible interaction with other proteolytic microbial strains was evaluated. A notorious synergistic effect was observed when B. cereus was inoculated with Pseudomonas sp. These brought a triple benefit, first, opening the possibility to produce technical enzymes at low cost, second, giving greater value to a food industry by-product, and third, reducing the environmental impact caused by the product removal directly into the environment. 相似文献
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IIntroductionTheuseofrestrlctlonfragmentlengthpolymorphism(RFLP)markershasgreatlyslmpllfledthegeneticanalysisofquantitativetraits,providingareliableandextensiveframeworkofquantltatlvemarkerstowhichquantltatlyetyaitIOCI(QTL)clnhilinked[‘].TodetectthelinkagebetwwenRFLPmarkersandPhenotyPlcvariationsoh-served,generallinearmodelofanalysisofvariance(ANOVA)hasbeenextensivelyusedL‘zJ.ByusingF、populations,thecompletegeneticInformation,thatIs,thethreegenotypesofageneticfact… 相似文献
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对长春花(Catharanthus roseus)纵生芽中长春朵灵和泻花碱进行薄层色谱分离。讨论了吸附剂、溶剂系统对长春朵灵和泻花碱比移值(R_t)的影响。给出了不同系统下长春朵灵和泻花碱的R_t的值。 相似文献
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