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The Mad and Myc basic domains are functionally equivalent 总被引:4,自引:0,他引:4
Nikiforov MA Popov N Kotenko I Henriksson M Cole MD 《The Journal of biological chemistry》2003,278(13):11094-11099
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P J Hurlin C Quéva P J Koskinen E Steingrímsson D E Ayer N G Copeland N A Jenkins R N Eisenman 《The EMBO journal》1995,14(22):5646-5659
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This paper describes an approach to seek for mouse c-Myc/Myn proteins-bound specific sequences among genomic DNA.cDNA fragment of myn gene was obtained through RT-PCR technique from RNA of NIH3T3 cells.DNA fragments encoding BR/HLH/LZ structure of Myc and Myn proteins were cloned in frame into pGEX-2T vector respectively.Fusion GST-Myc and GST-Myn synthesized in E.coli hosts showed affinity to CACGTG E-box DNA and subsequently interacted with genomic fragments prepared through whole-genome-PCR.A PCR-assisted procedure which combines protein-DNA interaction and affinity chromatography was designed to enrich Myc/Myn bound DNA.At least two genomic DNA fragments obtained exhibit specifical binding capacity to Myc/Myn complex but not to GST alone.Significance of the work and of the technique itself as well asidentification of the DNAs are discussed. 相似文献
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