Method for Growth and Purification of Bacteriophage 643 on Streptococcus lactis ML3 |
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| Authors: | David J. Lyttle and George B. Petersen |
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| Abstract: | The yield of bacteriophage 643 was increased by infecting cultures of Streptococcus lactis ML3 in late-log phase growth, harvesting the infected cells, and suspending them in fresh, phosphate-buffered minimal medium. The cells lysed after this treatment and produced high titers of bacteriophage. The phage particles were dissociated from debris by 2 M NaCl and purified by differential and CsCl band centrifugation. |
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