Mapping of rfa Genes in Salmonella typhimurium by ES18 and P22 Transduction and by Conjugation |
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| Authors: | Kuo T T Stocker B A |
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| Affiliation: | Department of Medical Microbiology, Stanford University School of Medicine, Stanford, California 94305. |
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| Abstract: | Loci termed rfa, determining biosynthesis of somatic lipopolysaccharide core, have been mapped in Salmonella typhimurium LT2. The smooth-specific phage P22 co-transduced two leaky rfa alleles with cysE and with pyrE; one of the leaky alleles is perhaps rfaG, and the other is an unidentified gene concerned with synthesis of the heptose-containing part of the core. The lipopolysaccharide-indifferent phage ES18 (or its variant ES18.h1) co-transduced rfaF, rfaG, rfaL, rfa(R-res-1), and rfa(R-res-2) alleles with cysE and with pyrE, at rates indicating the order cysE–rfaF–(rfa[R-res-1], rfa[r-res-2], rfaL)–rfaG–pyrE. One proven (and two suspected) rfaE alleles and five proven rfaH alleles were not co-transduced with cysE or pyrE. Hfr crosses indicated that the proven rfaE allele mapped between serA and strA. |
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