Conversion of Lactobacillus pentosus D-lactate dehydrogenase to a D-hydroxyisocaproate dehydrogenase through a single amino acid replacement |
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| Authors: | Tokuda Chizuka Ishikura Yoshiro Shigematsu Mayu Mutoh Hiroyuki Tsuzuki Shino Nakahira Yusaku Tamura Yusuke Shinoda Takeshi Arai Kazuhito Takahashi O Taguchi Hayao |
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| Affiliation: | Department of Applied Biological Science, Faculty of Science and Technology, Science University of Tokyo, Noda, Chiba 278-8510, Japan. |
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| Abstract: | The single amino acid replacement of Tyr52 with Leu drastically increased the activity of Lactobacillus pentosus NAD-dependent D-lactate dehydrogenase toward larger aliphatic or aromatic 2-ketoacid substrates by 3 or 4 orders of magnitude and decreased the activity toward pyruvate by about 30-fold, converting the enzyme into a highly active D-2-hydroxyisocaproate dehydrogenase. |
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