| 鲤鱼乳酸脱氢酶活性的QTL检测 |
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| 引用本文： | 毛瑞鑫,刘福军,张晓峰,张研,曹顶臣,鲁翠云,梁利群,孙效文. 鲤鱼乳酸脱氢酶活性的QTL检测[J]. 遗传, 2009, 31(4): 407-411. DOI: 10.3724/SP.J.1005.2009.00407 |
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| 作者姓名： | 毛瑞鑫 刘福军 张晓峰 张研 曹顶臣 鲁翠云 梁利群 孙效文 |
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| 作者单位： | 1. 中国水产科学院黑龙江水产研究所, 哈尔滨 150070;2. 上海海洋大学水产与生命学院, 上海 200090;3. 大连水产学院生命科学与技术学院, 大连 116023 |
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| 基金项目： | 国家重点基础研究发展规划(973计划) |
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| 摘 要： | 利用SSR分子标记结合“拟测交”策略, 以荷包红鲤抗寒品系和柏氏鲤远缘杂交所产生F1代为亲本及其F2代为作图群体, 应用Windows Map Manager 2.0软件的标记回归法进行乳酸脱氢酶(Lactate dehydrogenase, LDH)活性数量性状基因座单标记定位分析。在乳酸脱氢酶性状的标记回归研究中, 共发现12个标记与LDH性状关联, 对性状的贡献率为4.00%~10.00%, 其中HLJE222达到极显著水平(P<0.01)。为了进一步验证, 利用已有的生物信息学工具, 将与性状连锁的EST序列与公共数据库中的核酸序列进行同源搜索分析, 发现标记HLJE222的EST序列与斑马鱼DAZ associated protein 1的mRNA序列相匹配(相似性为94%), 与公共数据库中的已知蛋白序列进行相似性比较, 同样发现HLJE222的EST与斑马鱼DAZ associated protein 1相匹配(相似性为97%)。结果表明HLJE222位点与影响乳酸脱氢酶活性相关的基因连锁。
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| 关 键 词： | 鲤鱼 标记回归分析 拟测交策略 乳酸脱氢酶 EST-SSR |
| 收稿时间： | 2008-09-10 |
| 修稿时间： | 2008-11-16 |
Studies on quantitative trait loci related to activity of lactate dehydrogenase in common carp (Cyprinus carpio) |
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| MAO Rui-Xin,LIU Fu-Jun,ZHANG Xiao-Feng,ZHANG Yan,CAO Ding-Chen,LU Cui-Yun,LIANG Li-Qun,SUN Xiao-Wen. Studies on quantitative trait loci related to activity of lactate dehydrogenase in common carp (Cyprinus carpio)[J]. Hereditas, 2009, 31(4): 407-411. DOI: 10.3724/SP.J.1005.2009.00407 |
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| Authors: | MAO Rui-Xin LIU Fu-Jun ZHANG Xiao-Feng ZHANG Yan CAO Ding-Chen LU Cui-Yun LIANG Li-Qun SUN Xiao-Wen |
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| Affiliation: | 1. Heilongjiang River Fisheries Research Institute, Chinese Acadamy of Fishery Sciences, Harbin 150070, China;2. College of Fisheries and life Science, Shanghai Ocean University, Shanghai 200090, China;3. College of Life Science and Technology, Dalian Fisheries College, Dalian 116023, China |
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| Abstract: | Microsatellite markers were used to make marker regression analysis on activity of lactate dehydrogenase based on double pseudo-testcross strategy using Windows Map Manager2.0 software. The parents that came from the cross between progenies of Hebao-cold tolerance red carp and Barbless carp and F2 progenies were used as segregating popula-tions. For maker regression, a total of 12 markers associated with activity of lactate dehydrogenase were significant at P<0.05 and HLJE222 was significant at P<0.01. The variance explained by these loci, ranged from 4.00% to 10.00%. Lo-cus HLJE222 was closely linked to the gene related to activity of lactate dehydrogenase of common carp. For further identi-fication, EST-SSR markers were used to screen the protein and nucleotide database using bioinformatics tools in order to find the homologies. High sequence similarities of HLJE222 marker were observed with the nucleotide sequence of DAZ associated protein 1mRNA of zebrafish(94%), and protein sequence of DAZ associated protein 1(97%). DAZ protein is one of the short chain dehydrogenases, which is an important enzyme in the process of glucose metabolism in the organisms. This family contains a wide variety of dehydrogenases. This indicates that locus HLJE222 was closely linked to the gene associated with activity of lactate dehydrogenase of common carp. |
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| Keywords: | EST-SSR |
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