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Transformation in Escherichia coli: cryogenic preservation of competent cells. |
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| Authors: | D A Morrison |
| Abstract: | Escherichia coli cells prepared for transformation by treatment with cold 0.1 M CaCl2 remained viable and competent after storage at -82 degrees C in 15% glycerol; thawed-cell samples yielded up to 10(6) transformants per microgram of plasmid deoxyribonucleic acid. |
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