Differences in the ribosomes prepared from lactating and non-lactating bovine mammary gland |
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| Authors: | M. D. Herrington and A. O. Hawtrey |
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| Affiliation: | Department of Biochemistry, University College of Rhodesia, Salisbury, Rhodesia |
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| Abstract: | 1. Ribosomes prepared from bovine lactating mammary gland are able to synthesize protein, whereas similar preparations from non-lactating glands are not. Washing the ribosome suspensions through a medium containing 0.5m-ammonium chloride enhanced their ability to incorporate phenylalanine into polyphenylalanine. 2. Ribosomes isolated from non-lactating bovine mammary gland, in contrast with those from rat liver and lactating mammary gland, contained significant amounts of extraneous nucleases. These enzymes could be removed by washing with a medium A buffer containing 0.5m-ammonium chloride. 3. Only those ribosomes from functionally active tissues were able to bind polyuridylic acid and phenylalanyl-tRNA. |
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