| 茶小绿叶蝉成虫唾液细菌蛋白的鉴定 |
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| 引用本文： | 潘铖,钮羽群,夏露霞,伍春芳,景凯婷,程墙义,王梦馨,韩宝瑜. 茶小绿叶蝉成虫唾液细菌蛋白的鉴定[J]. 昆虫学报, 2021, 64(8): 929-942. DOI: 10.16380/j.kcxb.2021.08.005 |
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| 作者姓名： | 潘铖 钮羽群 夏露霞 伍春芳 景凯婷 程墙义 王梦馨 韩宝瑜 |
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| 作者单位： | (1. 中国计量大学生命科学学院, 浙江省生物计量及检验检疫技术重点实验室, 杭州 310018; 2. 安徽农业大学植物保护学院, 合肥 230000) |
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| 摘 要： | [目的]通过鉴定茶小绿叶蝉Empoasca flavescens成虫水状唾液蛋白中的细菌蛋白,解析叶蝉唾液的细菌种类.[方法]利用自制的收集装置和Parafilm膜夹营养液法对叶蝉成虫唾液进行收集,经超滤浓缩和电泳分离后,使用膜辅助样品制备技术法(filter-aided sample preparation,FASP...
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| 关 键 词： | 茶小绿叶蝉 唾液 细菌蛋白 共生菌 LC-MS/MS |
Identification of bacterial proteins in the saliva of adults of the tea green leafhopper,Empoasca flavescens (Hemiptera: Cicadellidae) |
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| PAN Cheng,NIU Yu-Qun,XIA Lu-Xia,WU Chun-Fang,JING Kai-Ting,CHENG Qiang-Yi,WANG Meng-Xin,HAN Bao-Yu. Identification of bacterial proteins in the saliva of adults of the tea green leafhopper,Empoasca flavescens (Hemiptera: Cicadellidae)[J]. Acta Entomologica Sinica, 2021, 64(8): 929-942. DOI: 10.16380/j.kcxb.2021.08.005 |
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| Authors: | PAN Cheng NIU Yu-Qun XIA Lu-Xia WU Chun-Fang JING Kai-Ting CHENG Qiang-Yi WANG Meng-Xin HAN Bao-Yu |
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| Affiliation: | (1. Zhejiang Provincial Key Laboratory of Biometrology and Inspection and Quarantine, College of Life Sciences, China Jiliang University, Hangzhou 310018, China; 2. School of Plant Protection, Anhui Agricultural University, Hefei 230000, China) |
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| Abstract: | : 【Aim】 To understand the bacteria species in the watery saliva of adults of the tea green leafhopper, Empoasca flavescens, by identifying the bacterial proteins in their saliva. 【Methods】 Saliva of tea green leafhopper adults was collected by the self-made collection device with treching two layers of Parafilm with sucrose diet. After concentration by ultrafiltration and electrophoretic separation, saliva protein solution was hydrolysated by filter-aided sample preparation (FASP) method. Saliva proteins were analyzed by liquid chromatography tandem mass spectrometry (LC-MS/MS). The bacterial proteins in saliva were searched against the UniProt database of bacterial protein by using Mascot 2.2. 【Results】 One hundred and forty-two proteins from 49 species of bacteria in 27 orders were identified. Bacterial proteins of Proteobacteria were the most abundant, with a total of 107 proteins, among which bacterial proteins of α-Proteobacteria and γ-Proteobacteria accounted for the most. Bacterial proteins of Firmicutes (28 proteins) were the second most abundant, belonging to Bacilli and Clostrida, respectively. These proteins are involved in amino acid, vitamin and energy metabolism. 【Conclusion】 The identified bacterial proteins may be important participants in the life cycle of leafhoppers. This study provides basic information for further study on the relationship among endosymbiotic bacteria, tea green leafhoppers and tea plants. |
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| Keywords: | Empoasca flavescens saliva bacterial protein endosymbiotic bacteria LC-MS/MS |
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