| 田间二化螟雄蛾对不同配比性信息素的嗅觉反应 及性信息素识别相关基因表达水平差异 |
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| 引用本文： | 刘天伟,陈运康,许春梅,郭荣,冯波,杜永均. 田间二化螟雄蛾对不同配比性信息素的嗅觉反应 及性信息素识别相关基因表达水平差异[J]. 昆虫学报, 2021, 64(5): 585-597. DOI: 10.16380/j.kcxb.2021.05.005 |
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| 作者姓名： | 刘天伟 陈运康 许春梅 郭荣 冯波 杜永均 |
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| 作者单位： | (1. 浙江大学农药与环境毒理研究所, 杭州 310058; 2. 湖南攸县植保植检站, 湖南攸县 412300; 3. 犍为县农业农村局, 四川犍为 614400; 4. 全国农业技术推广服务中心, 北京 100125; 5. 温州医科大学健康与环境生态研究所, 浙江温州 325035) |
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| 摘 要： | 【目的】本研究旨在从行为和嗅觉分子水平探究二化螟Chilo suppressalis雄蛾对性信息素嗅觉反应的地理种群差异及其机理，以明确该害虫田间种群雄成虫的嗅觉适应性及其特点。【方法】采用7种Z11-16∶Ald, Z9-16∶Ald和Z13-18∶Ald配比不同的三元性信息素诱芯(Z11-16∶Ald和Z9-16∶Ald配比分别为540 μg∶540 μg, 864 μg∶216 μg, 945 μg∶135 μg, 980 μg∶98 μg, 1 003 μg∶77 μg, 1 016 μg∶64 μg和1 045 μg∶35 μg，而Z13-18∶Ald含量保持不变)，在中国6省水稻田间诱捕二化螟雄成虫；利用实验室风洞测定864 μg∶216 μg, 980 μg∶98 μg和1 016 μg∶64 μg配比诱芯在田间诱捕的越冬代和第2代二化螟雄蛾分别对这3个配比的性信息素诱芯的行为反应；并采用RT-qPCR方法测定这7种性信息素配比不同的诱芯诱捕的雄蛾触角中二化螟雄蛾12个性信息素识别相关基因的表达水平。【结果】在田间试验中，Z11-16∶Ald和Z9-16∶Ald不同配比的性信息素诱芯对水稻二化螟雄蛾均有引诱作用，但不同配比引诱的二化螟雄蛾占比不同。6省诱芯中Z11-16∶Ald和Z9-16∶Ald的最佳配比不同，并且同一配比性信息素诱芯在不同省份引诱的二化螟雄蛾占比也不同。结果表明，不仅在不同地区诱芯的性信息素最佳配比不同，而且在不同地区不同配比性信息素的诱蛾量占比也不同。实验室风洞试验中，864 μg∶216 μg和1 016 μg∶64 μg配比诱芯田间诱捕雄蛾分别对864 μg∶216 μg和1 016 μg∶64 μg配比诱芯的行为反应均显著强于对其余两种配比诱芯，980 μg∶98 μg配比诱芯田间诱捕雄蛾对1 016 μg∶64 μg, 980 μg∶98 μg和864 μg∶216 μg配比诱芯的行为反应无显著差异。在RT-qPCR测定中，除GOBP1外，其余11个性信息素识别相关基因在Z11-16∶Ald和Z9-16∶Ald不同配比诱芯诱捕雄蛾触角中的表达水平均存在显著差异，其中PBP3, PBP4, PR1, PR2, PR4, PR5和PR6 7个基因的表达水平与性信息素配比之间存在显著的线性相关。【结论】本研究明确了中国二化螟不同地理种群性信息素嗅觉差异，不仅有助于提高该害虫的性诱防控效率，而且也有助于理解其嗅觉地理种群差异的成因。
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| 关 键 词： | 二化螟 性信息素 嗅觉 种群差异 诱捕 风洞 |
Variation in the olfactory response and expression levels of genes involved in sex pheromone recognition in male moths of field Chilo suppressalis (Lepidoptera: Crambidae) to sex pheromone blends at different ratios |
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| LIU Tian-Wei,CHEN Yun-Kang,XU Chun-Mei,GUO Rong,FENG Bo,DU Yong-Jun. Variation in the olfactory response and expression levels of genes involved in sex pheromone recognition in male moths of field Chilo suppressalis (Lepidoptera: Crambidae) to sex pheromone blends at different ratios[J]. Acta Entomologica Sinica, 2021, 64(5): 585-597. DOI: 10.16380/j.kcxb.2021.05.005 |
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| Authors: | LIU Tian-Wei CHEN Yun-Kang XU Chun-Mei GUO Rong FENG Bo DU Yong-Jun |
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| Affiliation: | (1. Institute of Pesticide and Environmental Toxicology, Zhejiang University, Hangzhou 310058, China; 2. Youxian County Station of Plant Protection, Youxian, Hunan 412300, China; 3. Agricultural and Rural Bureau of Qianwei County, Sichuan Province, Qianwei, Sichuan 614400, China; 4. National Extension and Service Center of Agricultural Technology, Beijing 100125, China; 5. Institute of Health and Environmental Ecology, Wenzhou Medical University, Wenzhou, Zhejiang 325035, China) |
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| Abstract: | 【Aim】 This study aims to explore the variation among geographical populations in the olfactory response of male moths of Chilo suppressalis to sex pheromone and the related mechanisms, so as to understand the olfactory adaptation of male adults in field populations of the pest and its characteristics. 【Methods】 The male moths of C. suppressalis were trapped in rice fields in six provinces of China by using seven lures containing 3-component sex pheromone blends of Z11-16∶Ald and Z9-16∶Ald at different ratios (540 μg∶540 μg, 864 μg∶216 μg, 945 μg∶135 μg, 980 μg∶98 μg, 1 003 μg∶77 μg, 1 016 μg∶64 μg and 1 045 μg∶35 μg) and Z13-18∶Ald with the constant content, the behavioral responses of male moths of the overwintering generation and the second generation trapped by the lures containing Z11-16∶Ald and Z9-16∶Ald at the ratios of 864 μg∶216 μg, 980 μg∶98 μg and 1 016 μg∶64 μg in the filed to the lures at these three ratios were determined by laboratory bioassay in the wind tunnel, and the expression levels of 12 genes involved in sex pheromone recognition in the antennae of male moths trapped by the seven lures containing sex pheromone blends at different ratios were detected by RT-qPCR. 【Results】 In the field tests, all sex pheromone lures containing different Z11-16∶Ald and Z9-16∶Ald ratios could attract male moths of C. suppressalis, but the proportions of male moths of C. suppressalis trapped by lures containing different Z11-16∶Ald and Z9-16∶Ald ratios were different. The optimal ratio of Z11-16∶Ald and Z9-16∶Ald was different among the six provinces, and the proportion of male moths of C. suppressalis trapped by lure containing the same Z11-16∶Ald and Z9-16∶Ald ratio was different among the six provinces. The results showed that not only the optimal ratios of sex pheromones of lures in different geographical locations were different, the proportions of moths attracted by sex pheromone blends at different ratios in different geographical locations were also different. In the laboratory wind tunnel bioassay, the behavioral responses of male moths trapped by lures containing Z11-16 Ald and Z9-16∶Ald at the ratios of 864 μg∶216 μg and 1 016 μg∶64 μg in the field to the lures at the ratios of 864 μg∶216 μg and 1 016 μg∶64 μg, respectively, were significantly higher than those to the lures at the other two ratios. However, the male moths trapped by the lure containing Z11-16∶Ald and Z9-16∶Ald at the ratio of 980 μg∶98 μg in the filed did not show significantly different behavioral responses to lures containing Z11-16∶Ald and Z9-16∶Ald at the ratios of 1 016 μg∶64 μg, 980 μg∶98 μg and 864 μg∶216 μg in the wind tunnel. In the RT-qPCR assay, except GOBP1, the expression levels of the other 11 genes involved in sex pheromone recognition in the antennae of the male moths of C. suppressalis trapped by lures containing Z11-16∶Ald and Z9-16∶Ald at different ratios were significantly different. In addition, there were significant linear correlations between the ratio of sex pheromone blends and the expression levels of genes PBP3, PBP4, PR1, PR2, PR4, PR5 and PR6.【Conclusion】 This study identified the variation in sex pheromone recognition of male moths among different geographical populations of C. suppressalis in China. The results not only contribute to the control of C. suppressalis, but also help to understand the mechanisms of its geographical variation in olfaction. |
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| Keywords: | Chilo suppressalis sex pheromone olfaction population variation trapping wind tunnel |
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