miRNA‐4317 suppresses human gastric cancer cell proliferation by targeting ZNF322 |
| |
| Authors: | Xiaoyi Hu Min Zhang Jiyu Miao Xiaofei Wang Chen Huang |
| |
| Affiliation: | 1. Department of Oral Maxillofacial Surgery, Stomatological Hospital, College of Stomatology, Xi'an Jiaotong University, Xi'an, Shaanxi, China;2. Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, College of Stomatology, Xi'an Jiaotong University, Xi'an, Shaanxi, China;3. Key Laboratory of Environment and Genes Related to Diseases, College of Medicine, Xi'an Jiaotong University, Xi'an, Shaanxi, China;4. College of Life Science, Yanan University, Yan'an, Shaanxi, China |
| |
| Abstract: | Studies have shown that miR‐4317 is dysregulated in tumor, but the biologic role of miR‐4317 in tumor development and progression remains unknown. The present study aimed to investigate the role of miR‐4317 in human gastric cancer. Quantitative real‐time PCR was used to quantify miR‐4317 expression levels in clinical gastric cancer specimens and cell lines. MTT, colony formation and cell cycle assays were performed to identify the contributions of miR‐4317 to cell proliferation in gastric cancer cell lines. The results showed that miR‐4317 was significantly decreased in 17 clinical gastric cancer specimens compared with adjacent non‐tumor stomach tissues. Forced expression of miR‐4317 suppressed gastric cancer cell proliferation and blocked S‐G2/M transition. Bioinformatics and dual‐luciferase reporter assays confirmed that ZNF322 is a direct target of miR‐4317. Silencing ZNF322 recapitulated the cellular and molecular effects seen upon miR‐4317 overexpression. These findings indicate that miR‐4317 represses the proliferation of gastric cancer cell, at least in part, by targeting and suppressing ZNF322 and that it may serve as a therapeutic target for gastric cancer treatment. |
| |
| Keywords: | gastric cancer miRNA‐4317 proliferation ZNF322 |
|
|
