Characteristics and restriction analysis of the 4-chlorobiphenyl catabolic plasmid, pSS50. |
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| Authors: | S W Hooper T C Dockendorff G S Sayler |
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| Abstract: | The plasmid pSS50 is a 53-kilobase self-transmissible plasmid of broad host range that has been isolated from several Alcaligenes and Acinetobacter species. This plasmid has previously been shown to mediate the mineralization of 4-chlorobiphenyl to carbon dioxide and water. Physical characterization of this plasmid by restriction analysis indicates that most hexanucleotide cleavage sites are clustered in a 5-kilobase region, leaving large regions without restriction sites. The paucity of restriction sites is not due to DNA methylation. |
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