| 鱼类核移植与重编程 |
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| 引用本文： | 王学耕,朱作言,孙永华,赵珏. 鱼类核移植与重编程[J]. 遗传, 2013, 35(4): 433-440. DOI: 10.3724/SP.J.1005.2013.00433 |
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| 作者姓名： | 王学耕 朱作言 孙永华 赵珏 |
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| 作者单位： | 1. 北京大学生命科学学院, 北京 100871 2. 中国科学院水生生物研究所, 淡水生态与生物技术国家重点实验室, 武汉 430072 |
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| 基金项目： | 斑马鱼细胞核移植发育再程序化研究项目(编号：30950001)资助 |
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| 摘 要： | 鱼类核移植是动物克隆研究的一个重要领域, 我国学者在上世纪60年代首创了鱼类的核移植研究。以斑马鱼为模式动物, 进行核移植与再程序化研究具有独特的优势。文章总结了鱼类细胞核移植研究的历史、斑马鱼核移植研究概况、以及影响核移植胚胎发育的因素, 特别是核移植胚胎基因组的表观遗传修饰, 如基因组DNA甲基化及组蛋白乙酰化和甲基化等的研究, 将有助于完善克隆技术并提高克隆的成功率, 推动克隆技术的广泛开展和应用。
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| 关 键 词： | 核移植 斑马鱼 再程序化 表观遗传修饰 |
| 收稿时间： | 2012-06-18 |
Nuclear transfer and reprogramming in fish |
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| Affiliation: | 1. College of Life Sciences, Peking University, Beijing 100871, China 2. State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China |
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| Abstract: | As an important sub-field in the study of animal cloning, fish nuclear transfer was first established in the early 1960s by Chinese embryologists. Due to its advantages, zebrafish has become a unique animal model to study the mystery of reprogramming in nuclear transfer. This article summarizes the history and current situation in fish nuclear transfer tech-nology and discusses the factors that may influence the development of the cloned embryos. A comprehensive understand-ing of the mechanism for epigenetic modification following nuclear transfer, such as genomic DNA methylation and histone acetylation and/or methylation, will likely increase the success rate and eventually lead to the future freedom of cloning technique. |
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| Keywords: | nuclear transfer zebrafish reprogramming epigenetic modification |
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