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| 光照下菜豆叶片抗氰呼吸与光合作用关系的分析北大核心CSCD | |
| 引用本文： | 冯汉青,管东东,焦青松,贾凌云,孙坤. 光照下菜豆叶片抗氰呼吸与光合作用关系的分析北大核心CSCD[J]. 植物生态学报, 2015, 39(1): 104-109. DOI: 10.17521/cjpe.2015.0011 |
| 作者姓名： | 冯汉青 管东东 焦青松 贾凌云 孙坤 |
| 作者单位： | 西北师范大学生命科学学院, 兰州 730070 |
| 基金项目： | 国家自然科学基金国家自然科学基金(31260059; 30900105)；教育部科学技术研究重点项目(211190)；甘肃省财政厅高校基本科研业务费项目 |
| 摘 要： | 为了进一步了解光照下植物呼吸作用的内在机理以及呼吸作用和光合作用的关系,该文研究了在光照下菜豆(Phaseolus vulgaris)叶片抗氰呼吸与光合作用的关系。研究发现,将黑暗下生长的菜豆幼苗叶片转到光照下10 h,总呼吸、抗氰呼吸以及抗氰呼吸在总呼吸中的比例均逐步上升;光照也导致了叶片叶绿体光合放氧和CO2固定的出现及其速率的增加,但光合放氧和CO2固定速率的增加均滞后于抗氰呼吸的增加。将黑暗下生长的叶片转到光照下之前用抗氰呼吸的抑制剂水杨基氧肟酸(SHAM)处理叶片,发现用SHAM处理并没有导致叶片在光照下光合放氧和CO2固定速率的明显变化,这也提示了黑暗下生长的叶片转至光照的过程中,抗氰呼吸和光合作用没有产生偶联。进一步研究发现,在黑暗中对叶片施加短时间的光照能够增加抗氰呼吸在总呼吸中的比例,但短时间的光照对叶片光合CO2固定速率没有影响。这些结果表明了光照对抗氰呼吸的诱导可以不依赖于光合作用,光照可能是作为一种直接的信号去诱导抗氰呼吸。 |
| 关 键 词： | 抗氰呼吸 光照 光合作用 |
| 收稿时间： | 2014-05-29 |
Analysis of the relationship between cyanide-resistant respiration and photosynthesis under light in Phaseolus vulgaris leaves |
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| FENG Han-Qing,GUAN Dong-Dong,JIAO Qing-Song,JIA Ling-Yun,SUN Kun. Analysis of the relationship between cyanide-resistant respiration and photosynthesis under light in Phaseolus vulgaris leaves[J]. Acta Phytoecologica Sinica, 2015, 39(1): 104-109. DOI: 10.17521/cjpe.2015.0011 | |
| Authors: | FENG Han-Qing GUAN Dong-Dong JIAO Qing-Song JIA Ling-Yun SUN Kun |
| Affiliation: | College of Life Sciences of Northwest Normal University, Lanzhou 730070, China |
| Abstract: | Aims In order to further understand the inner mechanism of plant respiration and the relationship between respiration and photosynthesis of plant under light, our objective was to reveal the relationship between cyanide-resistant respiration and photosynthesis in bean (Phaseolus vulgaris) leaves under light. Methods By exposing the dark-grown leaves to light for 10 h, changes in total respiration (Vt), the cyanide-resistant respiration (Valt), photosynthetic O2 evolution, and photosynthetic CO2 fixation, and the effects of inhibitor of the cyanide-resistant respiration on photosynthesis were measured and analyzed. We also measured and analyzed changes in the ratio of cyanide-resistant respiration, total respiration, and photosynthetic CO2 fixation when leaves in the dark were exposed to a brief period (10 min) of light. Important findings After exposing the dark-grown leaves to light for 10 h, Vt, Valt, and the value of Valt/Vt all increased. During the process, time-course analysis after the onset of illumination demonstrated that the induction of the cyanide-resistant respiration in light was prior to the formations of the photosynthetic O2 evolution and CO2 fixation. This observation indicates that the induction of the cyanide-resistant respiration by light is independent of photosynthesis. The dark-grown leaves pretreated with 1 mmol·L–1 salicylhydroxamic acid (SHAM; the inhibitor of the cyanide-resistant respiration) were exposed to illumination for 10 h, and SHAM did not result in apparent modification of the photosynthetic O2 evolution and CO2 fixation in the leaves when exposed to light. This observation also showed that there was no direct linkage between photosynthesis and the cyanide-resistant respiration when the dark-grown leaves were exposed to light. In addition, under the condition of darkness, 10 min of light illumination obviously increased the value of Valt/Vt but did not significantly affect the level of photosynthetic CO2 fixation. Results indicate that the induction of the cyanide-resistant respiration by light may be independent of photosynthesis. Light should have a direct influence on induction of the cyanide-resistant respiration. |
| Keywords: | cyanide-resistant respiration light illumination photosynthesis |
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