| 响应面优化塔拉种子多糖的超声波提取及其抗氧化性研究 |
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| 引用本文： | 赵寒梅,袁德成,杨逢建. 响应面优化塔拉种子多糖的超声波提取及其抗氧化性研究[J]. 植物研究, 2017, 37(4): 635-640. DOI: 10.7525/j.issn.1673-5102.2017.04.021 |
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| 作者姓名： | 赵寒梅 袁德成 杨逢建 |
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| 作者单位： | 东北林业大学森林植物生态学教育部重点实验室, 哈尔滨 150040 |
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| 基金项目： | 林业公益性行业科研专项（201404616） |
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| 摘 要： | 为获得塔拉多糖超声波提取的最佳工艺，利用中心组合实验设计原理，采用四因素三水平的响应面分析法，获得多元二次线性回归方程，以多糖提取率为响应值做响应面。并考察塔拉多糖的体外抗氧化活性。结果表明：塔拉多糖的最佳提取工艺条件为，超声辅助提取超声功率363 W，超声温度56℃，超声时间23 min，料液比1：17（g·mL-1），塔拉粗多糖的最大提取率（25.56%）与理论推测值（25.71%）相差较小。经纯化后塔拉多糖提取率为17.82%。以VC为阳性对照，对不同纯度的塔拉多糖进行抗氧化性的研究，发现其纯度越高，对ABTS自由基的清除能力越强。
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| 关 键 词： | 塔拉多糖 提取 响应面法 抗氧化性 |
| 收稿时间： | 2017-02-05 |
Optimization of Ultrasonic Extraction of Polysaccharides from Tara Seed and Its Antioxidant Activity |
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| ZHAO Han-Mei,YUAN De-Cheng,YANG Feng-Jian. Optimization of Ultrasonic Extraction of Polysaccharides from Tara Seed and Its Antioxidant Activity[J]. Bulletin of Botanical Research, 2017, 37(4): 635-640. DOI: 10.7525/j.issn.1673-5102.2017.04.021 |
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| Authors: | ZHAO Han-Mei YUAN De-Cheng YANG Feng-Jian |
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| Affiliation: | Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040 |
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| Abstract: | For the optimum ultrasonic extraction of Tara polysaccharide,a multiple quadratic regression was stablished using a 4-factor, 3-level Box-Behnken statistical design, and the response surface with the extraction rate of polysaccharides as the response were drawn. The antioxidant activities of Tara polysaccharide invitrowere tested. Theoptimumextractionconditions of Tara polysaccharide were:ultrasonic treatment at 56℃ for 23 min with ultrasonic power 363 W, and material to liquid ratio of 1:17 g·mL-1. The maximum extraction rate of Tara crude polysaccharides was 25.56%, less than the theoretical deduced value of 25.71%.The purified Tara polysaccharide extraction rate was 17.82%.Using VC as a positive control, the antioxidant activity of different purity Tara polysaccharide was measured, and it was found that its purity was high, and the ABTS radical scavenging ability was stronger. |
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| Keywords: | Tara polysaccharide extraction response surface analysis methodology (RSM) antioxidant activity |
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