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| 皮诺敛酸/左旋肉碱降低HepG2细胞脂质的最佳浓度配比研究 | |
| 引用本文： | 杨明非,张晶,苏雯,王鹏,张孙东,寇萍,焦骄,杨雨春,付玉杰. 皮诺敛酸/左旋肉碱降低HepG2细胞脂质的最佳浓度配比研究[J]. 植物研究, 2017, 37(6): 941-946. DOI: 10.7525/j.issn.1673-5102.2017.06.018 |
| 作者姓名： | 杨明非 张晶 苏雯 王鹏 张孙东 寇萍 焦骄 杨雨春 付玉杰 |
| 作者单位： | 1. 北日本制药株式会社产品研发室, 日本富山县中新川郡上市町若杉55号 930-0314; 2. 广东省珠海市斗门区公立医疗卫生机构管理中心, 珠海 519125; 3. 东北林业大学森林植物生态学教育部重点实验室, 哈尔滨 150040; 4. 吉林省林业科学院, 长春 130033 |
| 基金项目： | “十三五”国家重点研发计划项目（2016YFD0600805） |
| 摘 要： | 针对单一活性成分的减肥降脂效果较差，且容易出现耐药性等问题，本文筛选并优化了皮诺敛酸/左旋肉碱降低HepG2细胞脂质的最佳浓度配比，为皮诺敛酸降脂产品的开发提供基础数据。通过油酸诱导HepG2细胞成脂建立体外非酒精性脂肪肝模型。采用油红O染色法确定了皮诺敛酸/左旋肉碱降低HepG2细胞中脂滴的最佳浓度配比。实验结果表明0.5 mmol·L-1油酸诱导HepG2细胞脂肪变性效果最佳，皮诺敛酸（PLA）最佳降脂浓度为6.25 μmol·L-1，左旋肉碱（LC）最佳降脂浓度为250 μmol·L-1，皮诺敛酸/左旋肉碱（PLA/LC）最佳降脂浓度配比为1：40（μmol·L-1/μmol·L-1）。皮诺敛酸/左旋肉碱复配物可以显著降低HepG2细胞脂质，具有用量少，协同作用效果突出的优点，因此皮诺敛酸/左旋肉碱复配物在开发降脂减肥产品中具有良好的应用前景。 |
| 关 键 词： | 皮诺敛酸 左旋肉碱 复配 降脂 非酒精性脂肪肝 |
| 收稿时间： | 2017-03-15 |
The Optimized Proportion of Combination Pinoleic Acid/L-Carnitine Decrease Lipid in HepG2 |
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| YANG Ming-Fei,ZHANG Jing,SU Wen,WANG Peng,ZHANG Sun-Dong,KOU Ping,JIAO Jiao,YANG Yu-Chun,FU Yu-Jie. The Optimized Proportion of Combination Pinoleic Acid/L-Carnitine Decrease Lipid in HepG2[J]. Bulletin of Botanical Research, 2017, 37(6): 941-946. DOI: 10.7525/j.issn.1673-5102.2017.06.018 | |
| Authors: | YANG Ming-Fei ZHANG Jing SU Wen WANG Peng ZHANG Sun-Dong KOU Ping JIAO Jiao YANG Yu-Chun FU Yu-Jie |
| Affiliation: | 1. Research and Development Department of Kitanihon Pharmaceutical CO., No. 55, Kamiichi, Nakaniikawa District, Toyama, Japan 930-0314; 2. Management Center of Public Medical and Health Institutions in Doumen District, Zhuhai City, Guangdong Province, Zhuhai 519125; 3. Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040; 4. Forestry Academy of Jilin Province, Changchun 130033 |
| Abstract: | As the single active ingredient is poor and prone to drug resistance on effects of lose weight and lipid-lowering, so the study focuses on screening and optimizing the optimal concentration ratio of pinoleic acid/L-carnitine to reduce the lipid of HepG2 cell, which provides the basic data for the development of pinoleic acid lipid-lowering products. Oleic acid induced HepG2 cell adipogenesis, which was used to simulate the model of nonalcoholic fatty liver in vitro. The optimal concentration in combination of pinoleic acid/L-Carnitine decrease lipid was determinated by oil red O staining. The results show that the optimum concentration of oleic acid-induced HepG2 cell steatosis is 0.5 mmol·L-1.The optimal lipolysis concentration of pinoleic acid is 6.25 μmol·L-1. The optimal lipid metabolism concentration of L-Carnitine is 250 μmol·L-1. The best lipid-lowering ratio of pinoleic acid/L-carnitine(PLA/LC) is 1:40(μmol·L-1/μmol·L-1). The characteristics of pinoleic acid and L-Carnitine complexes decreased the doses and improved synergistic effect, Therefore, Combination of pinoleic acid/L-Carnitine has a potential prospectin in the development of lipid-lowering diet products. |
| Keywords: | pinoleic acid L-Carnitine complexes lipid-lowering nonalcoholic fatty liver disease |
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