Ethylene production by callus and suspension cells from cortex tissue of postclimacteric apples |
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| Authors: | Lieberman M Wang S Y Owens L D |
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| Affiliation: | Post Harvest Plant Physiology Laboratory and Cell Culture and Nitrogen Fixation Laboratory, Beltsville Agricultural Research Center, United States Department of Agriculture, Beltsville, Maryland 20705. |
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| Abstract: | Cortex tissue from postclimacteric `Golden Delicious' apples (Malus domestica, Borkh.) stored at 0 C for 9 months after harvest were induced to form callus in vitro. Cell suspension cultures were subsequently formed from calli. Of five media tested, only the medium of Schenk and Hildebrandt (Can J Bot 1972, 50: 192) and that of Uchimiya and Murashige (Plant Physiol 1974, 54: 936) allowed callus formation. During growth both the callus and cell cultures produced ethylene in a pattern which showed a rapid rise and then a fall as the culture grew. 14C-Labeled methionine was converted to labeled ethylene by the cell suspension cultures, which also could be inhibited from producing ethylene by a rhizobitoxine analog or free radical scavengers. Ethylene production in these cultures, like that in intact fruit tissue slices, could be stimulated by IAA or suppressed by N6-(γ,γ-dimethylallyl) adenosine and GA3. |
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