| 急、慢性运动对2型糖尿病大鼠脂肪PI3K/AKT/GLUT4通路的影响 |
| |
| 引用本文： | 衣雪洁,孙玉霞,姚婷婷,李晶,高畅,刘璐,曹师承,常波,张翠萍. 急、慢性运动对2型糖尿病大鼠脂肪PI3K/AKT/GLUT4通路的影响[J]. 中国应用生理学杂志, 2020, 36(1): 12-16. DOI: 10.12047/j.cjap.5802.2020.003 |
| |
| 作者姓名： | 衣雪洁 孙玉霞 姚婷婷 李晶 高畅 刘璐 曹师承 常波 张翠萍 |
| |
| 作者单位： | 1. 沈阳体育学院运动人体科学学院, 辽宁 沈阳 110102;2. 中国医科大学公共基础学院运动医学教研室, 辽宁 沈阳 110001 |
| |
| 基金项目： | 国家自然科学基金项目(30971414,81273096);辽宁省科学技术计划项目(2019-ZD-0516)。 |
| |
| 摘 要： | 目的:探讨急性和慢性运动对2型糖尿病(T2DM)大鼠脂肪组织明磷脂酰肌醇3激酶(PI3K)/蛋白激酶B(AKT)/葡萄糖运载体4(GLUT4)信号通路的影响。方法:15月龄SD雄性大鼠52只随机分为正常对照组(n=13)和高脂组(n=39),分别喂养普通和高脂饲料。8周后,高脂组体重>正常对照组20%,注射小剂量STZ后,血糖>16.7 mmol/l,造模成功。将糖尿病模型组随机分为糖尿病对照组(DC,n=13),糖尿病慢性运动组(DCE,n=13),糖尿病急性运动组(DAE,n=13)。DCE组进行8周的游泳运动,DAE组进行一次性游泳运动。测定血脂,血糖和血清胰岛素,Western blot法测定脂肪PI3K、AKT和GLUT4蛋白含量。结果:糖尿病组体重、血脂、血糖、胰岛素显著高于正常对照组(P均<0.01);高密度脂蛋白胆固醇(HDL-C)水平降低(P<0.05),脂肪组织中PI3K、AKT和GLUT4蛋白表达下降(P均<0.01)。糖尿病慢性运动组体重、血脂、血糖、胰岛素均出现显著性下降(P均<0.01);HDL-C升高(P<0.05...
|
| 关 键 词： | 2型糖尿病 脂肪 运动 磷脂酰肌醇3激酶/蛋白激酶B/葡萄糖转运载体4信号通路 大鼠 |
Effects of acute and chronic exercise on fat PI3K/AKT/GLUT4 signal pathway in type 2 diabetic rats |
| |
| YI Xue-jie,SUN Yu-xia,YAO Ting-ting,LI Jing,GAO Chang,LIU Lu,CAO Shi-cheng,CHANG Bo,ZHANG Cui-ping. Effects of acute and chronic exercise on fat PI3K/AKT/GLUT4 signal pathway in type 2 diabetic rats[J]. Chinese journal of applied physiology, 2020, 36(1): 12-16. DOI: 10.12047/j.cjap.5802.2020.003 |
| |
| Authors: | YI Xue-jie SUN Yu-xia YAO Ting-ting LI Jing GAO Chang LIU Lu CAO Shi-cheng CHANG Bo ZHANG Cui-ping |
| |
| Affiliation: | 1. School of Sports and Human Sciences, Shenyang Sport University, Shenyang 110102;2. Department of Sports Medicine, School of Public and Basic Sciences, China Medical University, Shenyang 110001, China |
| |
| Abstract: | Objective:To study the effects of acute and chronic exercise on phosphatidylinositol 3-hydroxy kinase(PI3K)/protein kinase B(AKT)/glucose transporter 4(GLUT4)signaling pathway in adipose tissue of rats with type 2 diabetes mellitus(T2DM)induced by high-fat diet and low-dose streptozotocin(STZ).Methods:A total of 52 SD male rats aged 15 months were randomly divided into normal control group(13)and high-fat group(39),and fed normal and high fat diets.After 8 weeks,the body weight of the high-fat group was higher 20%than that of the normal control group.After a small dose of STZ,the blood glucose level was>16.7 mmol/l,and the model was successfully established.The diabetic model group was randomly divided into a diabetic control group(DC,n=13),a diabetic chronic exercise group(DCE,n=13),and a diabetic acute exercise group(DAE,n=13).The DCE group underwent an 8-week swimming exercise and the DAE group performed a one-time swimming exercise.Blood lipids,blood glucose and serum insulin levels were measured,and the contents of fat PI3K,AKT and GLUT4 proteins were determined by Western blot method.Results:The levels of body weight,blood lipids,blood glucose and insulin in the diabetic group were significantly higher than those in the normal control group(P<0.01);high density liptein cholesterol(HDL-C)levels were decreased(P<0.05),and the expressions of PI3K,AKT and GLUT4 protein in adipose tissue were decreased(P<0.01).After 8 weeks of swimming training,the levels of body weight,blood lipids,blood glucose and insulin all were decreased significantly(P<0.01);while the level of HDL-C was increased(P<0.05),and the expressions of PI3K,AKT and GLUT4 protein were increased(P<0.01).After acute exercise,the levels of blood lipids,blood glucose and insulin were decreased(P<0.05);the level of HDL-C was increased(P<0.05),and the expression levels of fat PI3K,AKT and GLUT4 were increased significantly(P<0.05).Conclusion:①High fat diet combined with low-dose STZ induced damage to the PI3K/AKT pathway in adipose tissue of T2DM rats reduced insulin sensitivity.②Acute and chronic aerobic exercise can improve the disorder of glucose and lipid metabolism through PI3K/AKT pathway,and the chronic exercise is better than acute exercise. |
| |
| Keywords: | type 2 diabetes fat exercise phosphatidylinositol 3 kinase/protein kinase B/glucose transporter 4 rat |
| 本文献已被 CNKI 维普 等数据库收录！ |
| 点击此处可从《中国应用生理学杂志》浏览原始摘要信息 |
|
点击此处可从《中国应用生理学杂志》下载免费的PDF全文 |
|
