| 基于线粒体控制区和微卫星标记探讨秦岭细鳞鲑物种有效性 |
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| 引用本文： | 熊冬梅,蒙彦晓,张鑫淼,王继隆,冯广朋,邵俭,王立新. 基于线粒体控制区和微卫星标记探讨秦岭细鳞鲑物种有效性[J]. 水生生物学报, 2023, 47(5): 809-818. DOI: 10.7541/2022.2022.0418 |
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| 作者姓名： | 熊冬梅 蒙彦晓 张鑫淼 王继隆 冯广朋 邵俭 王立新 |
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| 作者单位： | 1.Department of Fisheries Science, College of Animal Science and Technology, Northwest A & F University, Yangling712100;2.Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin150070;3.East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai200090;4.College of Animal Science, Guizhou University, Guiyang550025; |
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| 基金项目： | National Natural Science Foundation of China, NSFC, (2021NY-002, 31302189, 32273138, 32273138和31302189)；Chinese Universities Scientific Fund, (2452020154)；Key Research and Development Projects of Shaanxi Province |
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| 摘 要： | 文章基于线粒体控制区基因序列和微卫星标记比较秦岭细鳞鲑(Brachymystax tsinlingensis Li)、黑龙江流域的尖吻细鳞鲑(Brachymystax lenok Pallas)和钝吻细鳞鲑(Brachymystax tumensis Mori)的分子遗传差异,为澄清其分类地位争议提供分子证据。结果表明:(1)扩增217个样本的mtDNA D-loop区序列,共获得45个单倍型,类群间无共享单倍型;基于单倍型构建的系统进化树显示三个细鳞鲑类群呈独立的支系;(2)基于14个呈多态性位点的遗传分化结果表明,秦岭细鳞鲑与尖吻或钝吻细鳞鲑之间的遗传距离均大于尖吻细鳞鲑和钝吻细鳞鲑之间的遗传距离;(3)基于线粒体D-loop和多态性微卫星位点计算出的遗传分化系数(FST)都远高于0.25表明三个类群间的遗传分化程度极高。这些结果表明,秦岭细鳞鲑与黑龙江流域细鳞鲑之间遗传分化程度高,结合前期发现秦岭细鳞鲑与黑龙江细鳞鲑类群有明显形态分化的研究结果及它们之间地理隔离已久的现状,研究初步判定秦岭细鳞鲑为独立物种,并建议以Brachymystax tsinlinge...
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| 关 键 词： | 线粒体控制区 微卫星标记 秦岭细鳞鲑 尖吻细鳞鲑 钝吻细鳞鲑 |
| 收稿时间： | 2022-10-12 |
THE VALIDITY OF SPECIES OF BRACHYMYSTAX TSINLINGENSIS LI BASED ON MITOCHONDRIA CONTROL REGION AND MICROSATELLITE |
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| Xiong D.-M.,Meng Y.-X.,Zhang X.-M.,Wang J.-L.,Feng G.-P.,Shao J.,Wang L.-X.. THE VALIDITY OF SPECIES OF BRACHYMYSTAX TSINLINGENSIS LI BASED ON MITOCHONDRIA CONTROL REGION AND MICROSATELLITE[J]. Acta Hydrobiologica Sinica, 2023, 47(5): 809-818. DOI: 10.7541/2022.2022.0418 |
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| Authors: | Xiong D.-M. Meng Y.-X. Zhang X.-M. Wang J.-L. Feng G.-P. Shao J. Wang L.-X. |
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| Abstract: | Qinling lenok (Brachymystax tsinlingensis Li), a national Class Ⅱ aquatic protected animal, is in urgent need of germplasm conservation due to the obvious decline of its wild population, however, since its subspecies was named in 1966, the status of its subspecies/species has been controversial until now. The unclear classification status seriously hinders the effective development of conservation work. Based on the mitochondrial control region gene sequence and microsatellite markers, we compared the molecular genetic differences between Qinling lenok and Heilongjiang lenok (Brachymystax lenok Pallas and Brachymystax tumensis Mori), to provide molecular evidence for clarifying the taxonomic status of Qinling lenok. The results showed as follows: (1) A total of 45 haplotypes were obtained by amplified mtDNA D-loop sequences of 217 samples, and no haplotypes were shared among above three groups. Phylogenetic tree based on haplotypes showed that each of the three groups of lenok was an independent clade. (2) The results of genetic differentiation based on 14 polymorphic loci showed that the genetic distance between Qinling lenok and B. lenok, Qinling lenok and B. tumensis was greater than the value between B. lenok and B. tumensis. (3) The genetic differentiation coefficient (FST) based on mitochondrial D-loop and polymorphic microsatellite loci analysis was higher than 0.25, indicating a high degree of genetic differentiation among the three groups. In the present study, a high degree of genetic differentiation among three groups were found. Combined with the previous results of obvious morphological differentiation between Qinling lenok and Heilongjiang lenok, which published by our team and the status of geographical isolation between Qinling and Heilongjiang rivers for a long time, we preliminarily determined that Qinling lenok is an independent species with Latin name Brachymystax tsinlingensis Li. It is suggested that Qinling lenok should be protected as an independent unit to avoid the destruction of germplasm resources caused by artificial introduction or hybridization. |
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| Keywords: | Brachymystax lenok Pallas Brachymystax tsinlingensis Li Brachymystax tumensis Mori Microsatellite marker Mitochondria control region |
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