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Identification of the New Pathogen (Stemphylium lycopersici) Causing Leaf Spot on Pepino (Solanum muricatum)
Authors:Abbas Nasehi  Jugah Kadir  Mehdi Nasr‐Esfahani  Farnaz Abed‐Ashtiani  Elham Golkhandan  Sadegh Ashkani
Affiliation:1. Department of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia, Serdang, Selangor Darul Ehsan, Malaysia;2. Isfahan Agricultural and Natural Resources Research Center, Plant Pests and Diseases Research Institute, Isfahan, Iran;3. Institute of Tropical Agriculture, Universiti Putra Malaysia, Serdang, Selangor, Malaysia;4. Department of Agronomy and Plant Breeding, Shahr‐e‐Rey Branch, Islamic Azad University, Tehran, Iran
Abstract:Pepino (Solanum muricatum var. pepino) plants were found affected by an extensive leaf spot caused by plant pathogenic fungi during a survey in the Cameron highlands, Pahang state, Malaysia. Symptomatic leaf samples were collected from infected pepino plants and cultivated on PDA medium, and the pathogen was isolated and purified; then, consequently, all isolates were identified as Stemphylium lycopersici on the basis of their cultural and morphological characteristics and combined sequences of the internal transcribed spacer (ITS) and glyceraldehyde‐3‐phosphate dehydrogenase (gpd) regions. A pathogenicity assay on detached leaves further confirmed that S. lycopersici causes leaf spot disease. To the best of our knowledge, this is the first report of S. lycopersici causing leaf spot on pepino in Malaysia and worldwide.
Keywords:leaf spot  pepino  phylogeny  Solanum muricatum var. pepino     Stemphylium lycopersici   
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