Effects of storage time on the motility,mortality and calcium levels of Atlantic salmon Salmo salar spermatozoa |
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| Authors: | J. Parodi G. Guerra M. Cuevas A. Ramírez‐Reveco F. Romero |
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| Affiliation: | 1. Laboratorio de Biología Celular Aplicada, Núcleo de Investigación en Producción Alimentaria, Escuela de medicina veterinaria, Facultad de recursos naturales, Universidad Católica of Temuco, Chile;2. Center of Neurosciences and Peptide Biology, Faculty of Medicine, Universidad de La Frontera, Temuco, Chile;3. Cryobiology and Spermatozoa Functionality Analysis Laboratory, Institute of Animal Sciences, Faculty of Veterinary Sciences, Universidad Austral de Chile, Valdivia, Chile |
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| Abstract: | This study estimates spermatozoa mortality, morphology, motility and intracellular calcium levels in Atlantic salmon Salmo salar milt after prolonged storage. Milt samples were preserved at 4° C for 25 days and then evaluated for mortality. Motility remained high for the first 3 days and the mortality was low during the first 5 days of storage. A decrease of >50% in calcium content was observed after 5 days of storage. When spermatozoa were activated, calcium levels increased >200% in relative fluorescence units (RFU); this rate of increase was lost when the samples were stored for extended periods of time and was only partially manifested in a zero calcium solution. The results suggest that in vitro storage of S. salar spermatozoa at 4° C for a period of 3 days preserves motility and limits mortality to levels similar to those of fresh spermatozoa. This method also maintains intracellular calcium storage critical for spermatozoa performance. |
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| Keywords: | conservation function milt viability |
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