| 寒冷对雌性C57BL/6小鼠动情周期的影响* |
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| 引用本文： | 赵海,李曦,张莉,武帅,张永强,李军,张格祥,杨丹凤. 寒冷对雌性C57BL/6小鼠动情周期的影响*[J]. 中国应用生理学杂志, 2019, 35(5): 454-456. DOI: 10.12047/j.cjap.5876.2019.098 |
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| 作者姓名： | 赵海 李曦 张莉 武帅 张永强 李军 张格祥 杨丹凤 |
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| 作者单位： | 1. 兰州大学公共卫生学院, 甘肃 兰州 730000; 2. 军事科学院军事医学研究院环境医学与作业医学研究所, 天津 300050; 3. 中国人民解放军32110部队卫生连, 黑龙江 黑河 164300 |
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| 基金项目： | BWS17J025;国家自然科学基金资助(21707165) |
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| 摘 要： | 目的:研究寒冷对雌性C57BL/6小鼠动情周期的影响。方法:12只雌性小鼠随机分为对照组、低温组,每组6只;低温组每天4℃暴露4 h,每天阴道涂片法观察小鼠动情状况,对照组饲养于常温动物房;每2 d称量体重,2周后心脏取血、子宫和卵巢,检测小鼠血清E2、FSH、LH、Prl、P水平,进行子宫、卵巢的组织病理学检查。结果:与对照组比较,低温组小鼠体重无显著性差异(P＞0.05),小鼠子宫脏器系数明显较低、动情间期明显延长(P＜0.01),血清FSH显著升高、Prl显著降低(P＜0.01),小鼠子宫腺管扩张,卵巢卵泡数量明显减少。结论:寒冷可使雌性C57BL/6小鼠动情周期延长,进而可能影响生殖功能。
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| 关 键 词： | 寒冷暴露 动情周期 C57BL/6小鼠 子宫 卵巢 |
| 收稿时间： | 2019-05-09 |
Effects of cold exposure on estrous cycle of female C57BL/6 mice |
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| ZHAO Hai,LI Xi,ZHANG Li,WU Shuai,ZHANG Yong-qiang,LI Jun,ZHANG Ge-xiang,YANG Dan-feng. Effects of cold exposure on estrous cycle of female C57BL/6 mice[J]. Chinese journal of applied physiology, 2019, 35(5): 454-456. DOI: 10.12047/j.cjap.5876.2019.098 |
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| Authors: | ZHAO Hai LI Xi ZHANG Li WU Shuai ZHANG Yong-qiang LI Jun ZHANG Ge-xiang YANG Dan-feng |
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| Affiliation: | 1. School of Public Health, Lanzhou University, Lanzhou 730000; 2. Institute of Environmental and Operational Medicine, Academy of Military Medical Sciences, Academy of Military Sciences, Tianjin 300050; 3. Chinese PLA 32110, Heihe, China |
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| Abstract: | Objective: To study the effects of cold exposure on estrous cycle of female C57BL/6 mice. Methods: Twelve healthy female C57BL/6 mice were randomly divided into two groups: control group and cold exposure group, 6 in each group. Cold exposure group was exposed to 4℃, 4 h per day, while control group stayed in normal conditions. Vaginal smears were used to observe the estrous cycle. After 2 weeks, blood and uteri were collected from each mouse after anesthetized and weighted. Serum levels of estradiol(E2), follicle-stimulating hormone(FSH), luteinizing hormone(LH), prolactin(Prl) and progesterone(P) were determined by using mouse ELISA kits. The uterus and ovary pathological slices were prepared to observe the structural changes. Results: Compared with the control group, body weight gain showed no significant differences (P＞0.05). The cold group had significant lower coefficients of uterus and the diestrus phase was significantly increased after cold exposure (P＜0.01). Serum level of FSH in cold group was higher and Prl was lower significantly (P＜0.01). Pathological examination of uterus and ovary showed that uterine glands of cold group were expanded and the amount of follicles was decreased significantly. Conclusion: Cold exposure might increase mouse estrous cycle and affect their reproductive function. |
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| Keywords: | cold exposure estrous cycle C57BL/6 mice uterus ovary |
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