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New restriction endonuclease CviRI cleaves DNA at TG/CA sequences. |
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| Authors: | A Jin Y Zhang Y Xia E Traylor M Nelson J L Van Etten |
| Abstract: | A new type II restriction endonuclease, CviRI, was isolated from virus XZ-6E infected chlorella cells. CviRI is the first restriction endonuclease to recognize the sequence 5'-TGCA-3' and cleaves DNA between the G and C residues to produce blunt-end termini. Methylation of the adenine or cytosine in 5'-TGCA-3' sequences prevents CviRI cleavage. Due to its sequence specificity, CviRI may be especially useful for detecting mutant alleles of many heritable human genetic diseases. |
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