The Xenopus laevis mitochondrial protein mtDBP-C cooperatively folds the DNA in vitro. |
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| Authors: | B Mignotte E Delain D Rickwood M Barat-Gueride |
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| Affiliation: | Laboratoire de Biologie Générale, Université de Paris-Sud, Orsay, France. |
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| Abstract: | The binding of the Xenopus laevis mitochondrial protein mtDBP-C to DNA was studied by equilibrium density banding, agarose gel electrophoresis and electron microscopy. The results obtained show that the mtDBP-C binds cooperatively to DNA irrespective of whether the DNA is supercoiled, relaxed or linear and it induces the formation of superhelical turns locally leading to the formation of a highly folded structure. It appears that this protein could be involved in the compaction of DNA in the mitochondrial nucleoid. |
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