Gap junction channel protein innexin 2 is essential for epithelial morphogenesis in the Drosophila embryo |
| |
| Authors: | Bauer Reinhard Lehmann Corinna Martini Julia Eckardt Franka Hoch Michael |
| |
| Affiliation: | Institut für Molekulare Physiologie und Entwicklungsbiologie der Universit?t Bonn, Abt. für Molekulare Entwicklungsbiologie, 53115 Bonn, Germany. |
| |
| Abstract: | Direct communication of neighboring cells by gap junction channels is essential for the development of tissues and organs in the body. Whereas vertebrate gap junctions are composed of members of the connexin family of transmembrane proteins, in invertebrates gap junctions consist of Innexin channel proteins. Innexins display very low sequence homology to connexins. In addition, very little is known about their cellular role during developmental processes. In this report, we examined the function and the distribution of Drosophila Innexin 2 protein in embryonic epithelia. Both loss-of-function and gain-of-function innexin 2 mutants display severe developmental defects due to cell death and a failure of proper epithelial morphogenesis. Furthermore, immunohistochemical analyses using antibodies against the Innexins 1 and 2 indicate that the distribution of Innexin gap junction proteins to specific membrane domains is regulated by tissue specific factors. Finally, biochemical interaction studies together with genetic loss- and gain-of-function experiments provide evidence that Innexin 2 interacts with core proteins of adherens and septate junctions. This is the first study, to our knowledge, of cellular distribution and protein-protein interactions of an Innexin gap junctional channel protein in the developing epithelia of Drosophila. |
| |
| Keywords: | |
| 本文献已被 PubMed 等数据库收录！ |
|
