A missense mutation accounts for the defect in the glycerol-3-phosphate acyltransferase expressed in the plsB26 mutant |
| |
| Authors: | Heath R J Rock C O |
| |
| Affiliation: | Department of Biochemistry, St Jude Children's Research Hospital, Memphis, Tennessee 38101, USA. |
| |
| Abstract: | The sn-glycerol-3-phosphate acyltransferase (plsB) catalyzes the first step in membrane phospholipid formation. A conditional Escherichia coli mutant (plsB26) has a single missense mutation (G1045A) predicting the expression of an acyltransferase with an Ala349Thr substitution. The PlsB26 protein had a significantly reduced glycerol-3-phosphate acyltransferase specific activity coupled with an elevated Km for glycerol-3-phosphate. |
| |
| Keywords: | |
| 本文献已被 PubMed 等数据库收录！ |
|
