A critical element of the light‐induced quaternary structural changes in YtvA‐LOV |
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| Authors: | Jayoung Moon Seung‐Goo Lee Young‐Ger Suh Bong‐Jin Lee Jeeyeon Lee |
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| Affiliation: | 1. College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, Seoul, Korea;2. Synthetic Biology and Bioengineering Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Korea |
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| Abstract: | YtvA, a photosensory LOV (light‐oxygen‐voltage) protein from Bacillus subtilis, exists as a dimer that previously appeared to undergo surprisingly small structural changes after light illumination compared with other light‐sensing proteins. However, we now report that light induces significant structural perturbations in a series of YtvA‐LOV domain derivatives in which the Jα helix has been truncated or replaced. Results from native gel analysis showed significant mobility changes in these derivatives after light illumination; YtvA‐LOV without the Jα helix dimerized in the dark state but existed as a monomer in the light state. The absence of the Jα helix also affected the dark regeneration kinetics and the stability of the flavin mononucleotide (FMN) binding to its binding site. Our results demonstrate an alternative way of photo‐induced signal propagation that leads to a bigger functional response through dimer/monomer conversions of the YtvA‐LOV than the local disruption of Jα helix in the As‐LOV domain. |
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| Keywords: | light LOV (light‐oxygen‐voltage) domain mobility changes optogenetic applications |
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