Effect of a Gene-Specific Suppressor Mutation (das) on DNA Synthesis of Gene 46-47 Mutants of Bacteriophage T4D |
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| Authors: | D. B. Shah and Hillard Berger |
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| Affiliation: | aDepartment of Cell Biology, University of Kentucky Medical School, Lexington, Kentucky 40506;Department of Biology, The Johns Hopkins University, Baltimore, Maryland 21218 |
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| Abstract: | Mutants in genes 46 and 47 of bacteriophage T4 exhibit early cessation of DNA synthesis, inability to form a normal rapidly sedimenting DNA intermediate (200S), reduced genetic recombination, and reduced viable phage production. A gene-specific suppressor mutation called das partially restores many of the pleiotropic effects of gene 46-47 mutants (13). Our results indicate that this partial suppression by das is associated with (i) the synthesis of a small fraction of DNA containing long single chains not detectable in 46-47 infection and (ii) a decrease in an "early" function which participates in the degradation of DNA synthesized in the absence of 46-47 functions. However, das does not restore the formation of a normal rapidly sedimenting (200S) DNA intermediate. |
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