Protein and Ribonucleic Acid Synthesis during Germination of Uredospores |
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| Authors: | Trocha P Daly J M |
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| Affiliation: | Department of Biochemistry and Nutrition, University of Nebraska, Lincoln, Nebraska 68503. |
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| Abstract: | Existing evidence suggests that there is no net synthesis of protein during germination of spores of some obligate parasites. The problem was reinvestigated with enzymatic hydrolysis, rather than acid hydrolysis or solubility properties, to estimate changes in protein and RNA. Procedures are detailed for the use of uniformly labeled 14C-uredospores for the isolation and quantitative assay of amino acids and nucleosides resulting from hydrolysis. |
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