反流性食管炎患者口腔菌群变化及其与幽门螺杆菌感染的关系 Changes of oral flora in patients with reflux esophagitis and its relationship with <i>Helicobacter pylori</i> infection期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

反流性食管炎患者口腔菌群变化及其与幽门螺杆菌感染的关系

引用本文：	王利丽, 李兰花, 李文伦, 等. 反流性食管炎患者口腔菌群变化及其与幽门螺杆菌感染的关系[J]. 中国微生态学杂志, 2022, 34(12): 1432-1435. doi: 10.13381/j.cnki.cjm.202212012

作者姓名：	王利丽李兰花李文伦栗欣李艳慧

作者单位：	南京同仁医院消化内科，江苏 211102

摘要：	目的探讨反流性食管炎（RE）患者口腔菌群变化及其与幽门螺杆菌（H. pylori）感染的关系，为该类患者的治疗提供参考。方法选择南京同仁医院2019年5月至2021年5月收治的140例RE患者为研究对象，将A、B级RE患者纳入AB组，C、D级纳入CD组；同时将患者分为合并H. pylori感染组和未合并H. pylori感染组。以一次性无菌收集管收集患者唾液标本，使用Illumina Hiseq 2500高通量测序平台对合格文库进行双端测序。结果 AB组患者H. pylori阳性率（16.67%）低于CD组（45.45%）。AB组患者口腔菌群Chao 1指数和Shannon指数均高于CD组，Simpson指数低于CD组（均P＜0.05）。未合并H. pylori感染组患者口腔菌群Chao 1指数和Simpson指数高于合并H. pylori感染组，Shannon指数低于合并H. pylori感染组（均P＜0.05）。未合并H. pylori感染组患者口腔放线菌门、放线菌属相对丰度高于合并H. pylori感染组，拟杆菌门相对丰度低于合并H. pylori感染组（均P＜0.05）。结论 RE病情较重者和合并H. pylori感染者表现为口腔菌群α多样性降低和门、属水平菌群相对丰度改变，合并H. pylori感染对RE患者病情和口腔菌群会产生一定影响。
关键词：	反流性食管炎口腔菌群幽门螺杆菌 α多样性相对丰度
收稿时间：	2022-05-17
修稿时间：	2022-08-15
*Changes of oral flora in patients with reflux esophagitis and its relationship with Helicobacter pylori* infection**

WANG Li-li, LI Lan-hua, LI Wen-lun, et al. Changes of oral flora in patients with reflux esophagitis and its relationship with Helicobacter pylori infection[J]. Chinese Journal of Microecology, 2022, 34(12): 1432-1435. doi: 10.13381/j.cnki.cjm.202212012

Authors:	WANG Li-li LI Lan-hua LI Wen-lun LI Xin LI Yan-hui

Affiliation:	Department of Gastroenterology, Nanjing Tongren Hospital, Nanjing , Jiangsu 211102, China

Abstract:	Objective To observe the changes of oral microecology in patients with reflux esophagitis (RE) and its relationship with Helicobacter pylori (H. pylori) infection. Methods A total of 140 RE patients admitted to Nanjing Tongren Hospital from May 2019 to May 2021 were selected as the subjects. The RE patients with grades A and B were included in the AB group, while those with grades C and D were included in the CD group. Upon recombination, the patients were divided into H. pylori infection group and non-H. pylori infection group. Saliva samples were collected in disposable sterile collection tubes. Eligible libraries were paired-end sequenced using the Illumina Hiseq 2500 high-throughput sequencing platform. Results The positive rate of H. pylori in AB group was lower than that in CD group (16.67% vs 45.45%). The oral flora Chao 1 index and Shannon index in the AB group were higher, while the Simpson index was lower than in the CD group, respectively (all P＜0.05). The oral flora Chao 1 index and Simpson index in non-H. pylori group were higher, while the Shannon index was lower than in H. pylori infection group, respectively. The relative abundances of Actinobacteria and Actinomyces in non-H. pylori group were higher, while that of Bacteroidetes was lower than in the H. pylori infection group respectively (all P＜0.05). Conlclusion Severe RE patients and patients with H. pylori infection showed a decrease in the α diversity of oral colonies and changes in the relative abundance at phylum and genus levels. Concommitant H. pylori infection had a certain impact on RE disease and oral microecological communities.

Keywords:	Reflux esophagitis Oral microecology Helicobacter pylori Alpha diversity Relative abundance

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