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| 绝经综合征患者肠道菌群特征 | |
| 引用本文： | 刘雅倩, 周英, 姚珮珣, 等. 绝经综合征患者肠道菌群特征[J]. 中国微生态学杂志, 2023, 35(8): 936-942, 949. doi: 10.13381/j.cnki.cjm.202308010 |
| 作者姓名： | 刘雅倩 周英 姚珮珣 赖雅薇 毛婷 黄燕梅 梁静涛 朱敏 宗云 郎建英 张莹轩 |
| 作者单位： | 1. 东莞市妇幼保健院妇科，广东 523057; 2. 广州中医药大学第一附属医院妇儿中心; 3. 广州中医药大学第一临床医学院; 4. 广州中医药大学基础医学院; 5. 南方医科大学珠江医院检验科 |
| 基金项目： | 广州中医药大学第一附属医院创新强院工程（2019IIT17） |
| 摘 要： | 研究绝经综合征患者的肠道菌群特征，为该类患者的治疗提供参考。 选取77例绝经综合征患者为病例组（P组），24例绝经前后健康女性为健康对照组（H组），采用16S rRNA基因测序技术检测两组对象肠道菌群结构，计算临床参数与肠道菌群的Spearman相关系数并采用PICRUSt2进行菌群的功能预测。 两组对象肠道菌群的alpha和beta多样性差异均无统计学意义（均 绝经综合征患者存在肠道菌群结构紊乱，表现为与性激素水平相关的聚集杆菌、动物双歧杆菌动物亚种和吉氏不动杆菌丰度降低，同时在绝经综合征患者中存在独特的代谢途径。 |
| 关 键 词： | 绝经综合征 肠道菌群 16S rRNA基因测序 |
| 收稿时间： | 2022-09-01 |
| 修稿时间： | 2023-03-18 |
The characteristics of gut microbes in patients with menopausal syndrome |
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| LIU Yaqian, ZHOU Ying, YAO Peixun, et al. The characteristics of gut microbes in patients with menopausal syndrome[J]. Chinese Journal of Microecology, 2023, 35(8): 936-942, 949. doi: 10.13381/j.cnki.cjm.202308010 | |
| Authors: | LIU Yaqian ZHOU Ying YAO Peixun LAI Yawei MAO Ting HUANG Yanmei LIANG Jingtao ZHU Min ZONG Yun LANG Jianying ZHANG Yingxuan |
| Affiliation: | 1. Gynecology Department of Dongguan Maternal and Child Health Care Hospital, Dongguan, Guangdong, 523057, China |
| Abstract: | ObjectiveTo observe the characteristics of gut microbes in menopausal syndrome (MS) patients, providing a reference for the treatment. Methods A total of 77 patients with MS were selected as the case group (P group), with 24 healthy women before and after menopause as the healthy control group (H group). 16S rRNA gene sequencing was used to measure the structure of gut microbes in the feces of the subjects. Spearman correlation coefficient between clinical parameters and gut microbes was calculated and PICRUSt2 was used to predict the function. ResultsThere were no significant differences in alpha and beta diversity of gut microbes between the two groups (all P＞0.05). There were 14 species with significant differences between the two groups (LDA score＞2.0) according to LEfSe analysis. Spearman′s correlation analysis showed that estradiol had a positive correlation with Aggregatibacter segnis, Bifidobacterium animalis subsp. animalis, Acinetobacter guillouiae (all P＜0.05), these three species were enriched in healthy women at menopause, while follicule-stimulating hormone and luteinizing hormone had a negative correlation with them (all P＜0.05). According to functional prediction by PICRUSt2, KEGG L3 metabolic pathways relevant to cardiovascular disease and carbohydrate metabolism were enriched in the MS patients (all P＜0.05). ConclusionThere was gut microbiota dysbiosis in patients with MS, which is reflected in the decrease of the abundance of Aggregatibacter segnis, Bifidobacterium animalis subsp. animalis and Acinetobacter guillouiae related to the level of sex hormones. In MS individuals, species with altered abundances and unique functional pathways were found. |
| Keywords: | Menopausal syndrome Gut microbes 16S rRNA gene sequencing |
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