Lateral ligand-receptor interactions on membranes probed by simultaneous fluorescence-interference detection |
| |
| Authors: | Gavutis Martynas Lata Suman Lamken Peter Müller Pia Piehler Jacob |
| |
| Affiliation: | Institute of Biochemistry, Biocenter N210 Johann Wolfgang Goethe-University, Frankfurt am Main, Germany. |
| |
| Abstract: | We describe an experimental approach for studying ligand-receptor interactions in the plane of the membrane. The extracellular domains of the type I interferon receptor subunits ifnar1-EC and ifnar2-EC were tethered in an oriented fashion onto solid-supported, fluid lipid bilayers, thus mimicking membrane anchoring and lateral diffusion of the receptor. Ligand-induced receptor assembling was investigated by simultaneous total internal reflection fluorescence spectroscopy and reflectance interferometry (RIf). Based on a rigorous characterization of the interactions of fluorescence-labeled IFNalpha2 with each of the receptor subunits, the dynamics of the ternary complex formation on the fluid lipid bilayer was addressed in further detail making use of the features of the simultaneous detection. All these measurements supported the formation of a ternary complex in two steps, i.e., association of the ligand to ifnar2-EC and subsequent recruitment of ifnar1-EC on the surface of the membrane. Based on the ability to control and quantify the receptor surface concentrations, equilibrium, and rate constants of the interaction in the plane of the membrane were determined by monitoring ligand dissociation at different receptor surface concentrations. Using mutants of IFNalpha2 binding to ifnar2-EC with different association rate constants, the key role of the association rate constants for the assembling mechanism was demonstrated. |
| |
| Keywords: | ifnar, human type I interferon receptor EC, extracellular domain IFN, human type I interferon wt, wild-type tl, tagless RIf, reflectance interferometry TIRFS, total internal reflection fluorescence spectroscopy SPR, surface plasmon resonance AF488, Alexa Fluor 488 OG488, Oregon Green 488 |
| 本文献已被 ScienceDirect PubMed 等数据库收录！ |
|
