Recognition imaging with a DNA aptamer |
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| Authors: | Lin Liyun Wang Hongda Liu Yan Yan Hao Lindsay Stuart |
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| Affiliation: | Department of Physics, Biodesign Institute, Arizona State University, Tempe, AZ, USA. |
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| Abstract: | We have used a DNA-aptamer tethered to an atomic force microscope probe to carry out recognition imaging of IgE molecules attached to a mica substrate. The recognition was efficient (approximately 90%) and specific, being blocked by injection of IgE molecules in solution, and not being interfered with by high concentrations of a second protein. The signal/noise ratio of the recognition signal was better than that obtained with antibodies, despite the fact that the average force required to break the aptamer-protein bonds was somewhat smaller. |
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