Isolation and characterization of mitochondrial deoxyribonucleic acid of Acanthamoeba castellanii |
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| Authors: | Navagnana S. Hettiarachchy and I. Gwyn Jones |
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| Affiliation: | Department of Biochemistry, University of Hull, Hull HU6 7RX, U.K. |
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| Abstract: | DNA was prepared from isolated mitochondria of Acanthamoeba castellanii and was shown to behave as a single component in density gradients, on ;melting' and on renaturation. From measurements of renaturation kinetics, sedimentation coefficient and electron micrographs the genome size of the mitochondrial DNA was calculated to be about 3.4x10(7) daltons. A small proportion of the preparations could be isolated as relaxed circular molecules of mean contour length 16.2mum. |
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