Evidence of two isomers of phosphatidylinositol in plant tissue |
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Authors: | Murthy P P Pliska-Matyshak G Keranen L M Lam P Mueller H H Bhuvarahamurthy N |
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Affiliation: | Department of Chemistry and Phytotechnology Research Center, Michigan Technological University, Houghton, Michigan 49931-1295. |
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Abstract: | The structure of phosphatidylinositol in barley (Hordeum vulgare) aleurone layers was investigated by chemical degradation. In vivo myo-[2-3H]inositol-labeled phosphatidylinositol was first converted to glycerophosphoinositol and, subsequently, after removal of the glycerol moiety, to inositol monophosphate. Here, we present data that show that, in addition to the commonly occurring 1,2-diacylglycero-3-(d-myo-inositol-1-phosphate), barley aleurone cells contain a novel second isomer of phosphatidylinositol that differs in structure of the head group. |
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