Chromium Differentially Affects Hydrogen Peroxide Distribution in Primary and Adventitious Roots of Arabidopsis thaliana L. |
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| Authors: | Aarón Giovanni Munguía-Rodríguez José López-Bucio Gerardo Rangel Sánchez León Francisco Ruiz-Herrera Yazmín Carreón-Abud Miguel Martínez-Trujillo |
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| Affiliation: | 1 Instituto de Investigaciones Químico-Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Edificio B3, Ciudad Universitaria, C. P, 58030, Morelia, Michoacán, México2 Facultad de Biología, Universidad Michoacana de San Nicolás de Hidalgo. Edificio R, Ciudad Universitaria, C. P, 58030 Morelia, Michoacán, México |
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| Abstract: | The post-embryonic growth of the Arabidopsis thaliana root system can be modified by different types of stress, such as sublethal concentrations of metals, which may induce the production of reactive oxygen species (ROS). In this study,the effects of different concentrations of potassium chromate (K₂CrO4) on the distribution and relative quantity of hydrogen peroxide (H2O2) were determined inprimary and adventitious roots in A. thaliana HyPer line seedlings. This line has a biosensor that specifically reports H2O2 levels within tissues as fluorescence. Primary root growth was inhibited at 100 μM Cr (VI); in contrast, adventitious rootformation was induced over the main root growth axis. These structures proliferated from 100-160 μM Cr (VI), and much higher concentrations (180-200 μM) of K₂CrO4 were required to affect their growth. The H2O2 distributions were observed in the columella and lateral root cap of primary roots of plants grown in medium lacking dichromate, but following the development of toxicity symptoms, H2O2 changed its distribution to the meristem and differentiation zones. Conversely, adventitious roots had comparable H2O2 distribution patterns in untreated plantsand those exposed to Cr (VI) supplementation. Thus, differential H2O2 distribution correlates with the resistance of adventitious roots, but not primary roots, to dichromate and underlies cell reorganization at the apex to support growth. |
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| Keywords: | Chromate hydrogen peroxide root growth meristem ion toxicity adaptation |
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