A convenient spectrophotometric assay for the determination of l-ergothioneine in blood |
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| Authors: | Jan Carlsson Marek P. J. Kierstan Keith Brocklehurst |
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| Affiliation: | Department of Biochemistry and Chemistry, St. Bartholomew''s Hospital Medical College, University of London, Charterhouse Square, London EC1M 6BQ, U.K. |
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| Abstract: | 1. A convenient spectrophotometric assay for the determination of l-ergothioneine in solution including deproteinized blood haemolysate was developed. 2. The method consists of deproteinization by heat precipitation and Cu(2+)-catalysed oxidation of thiols such as glutathione and of l-ascorbic acid, both in alkaline media, and titration of l-ergothioneine (which is not oxidized under these conditions) by its virtually instantaneous reaction with 2,2'-dipyridyl disulphide at pH1. 3. This method and the results obtained with it for the analysis of human, horse, sheep and pig blood are compared with existing methods of l-ergothioneine analysis and the results obtained thereby. |
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