首页 | 本学科首页   官方微博 | 高级检索  
     


A convenient spectrophotometric assay for the determination of l-ergothioneine in blood
Authors:Jan Carlsson   Marek P. J. Kierstan     Keith Brocklehurst
Affiliation:Department of Biochemistry and Chemistry, St. Bartholomew''s Hospital Medical College, University of London, Charterhouse Square, London EC1M 6BQ, U.K.
Abstract:1. A convenient spectrophotometric assay for the determination of l-ergothioneine in solution including deproteinized blood haemolysate was developed. 2. The method consists of deproteinization by heat precipitation and Cu(2+)-catalysed oxidation of thiols such as glutathione and of l-ascorbic acid, both in alkaline media, and titration of l-ergothioneine (which is not oxidized under these conditions) by its virtually instantaneous reaction with 2,2'-dipyridyl disulphide at pH1. 3. This method and the results obtained with it for the analysis of human, horse, sheep and pig blood are compared with existing methods of l-ergothioneine analysis and the results obtained thereby.
Keywords:
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号