| Cd2+、Al3+作用下蚕豆UDS与微核相关性分析及高等植物UDS技术初探 |
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| 引用本文： | 常学秀,文传浩,王焕校. Cd2+、Al3+作用下蚕豆UDS与微核相关性分析及高等植物UDS技术初探[J]. 应用生态学报, 1999, 10(5): 596-598 |
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| 作者姓名： | 常学秀 文传浩 王焕校 |
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| 作者单位： | 云南大学生物系, 昆明650091 |
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| 基金项目： | 国家自然科学基金资助项目 |
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| 摘 要： | 为探讨金属离子对高等植物非按期DNA合成(UnscheduledDNASythesis,简称UDS)和微核(MCN)的诱导作用、二者之间的关联性以及利用高等植物UDS技术检测环境诱变物的可行性,利用3HTdR前体掺入法研究了Cd2+、Al3+作用下蚕豆的UDS效应。结果表明,Cd2+、Al3+均能不同程度地诱导蚕豆UDS和MCN的发生;UDS量与微核率(MCNF)之间呈负相关(r<0),但相关不显着(|r|0.05),且二者间的相关程度在Cd2+和Al3+两种金属离子作用下没有显着差别(P>0.05);利用高等植物UDS技术检测环境诱变物质,在一定受检物剂量范围内是可靠的,但超过这个剂量范围,UDS技术无法检出.
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| 关 键 词： | 金属离子 UDS 微核 相关分析 高等植物UDS技术 |
| 收稿时间： | 1997-11-20 |
Correlation analysis between UDS and MCN in Vicia faba treated with Cd2+ and Al3+ and UDS technique in higher plants |
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| Chang Xuexiu,Wen Chuanhao,Wang Huanxiao. Correlation analysis between UDS and MCN in Vicia faba treated with Cd2+ and Al3+ and UDS technique in higher plants[J]. The journal of applied ecology, 1999, 10(5): 596-598 |
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| Authors: | Chang Xuexiu Wen Chuanhao Wang Huanxiao |
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| Affiliation: | Biology Department, Yunnan University, Kunming 650091 |
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| Abstract: | With the method of inserting 3H TdR into DNA, this paper studied the inductive effect of Cd 2 and Al 3 on unscheduled DNA synthesis (UDS) and micronucleus (MCN) in Vicia faba , the correlation between UDS and MCN, and the feasibility of using higher plant UDS test as the indicator to detect environmental mutagens. The results indicated that both Cd 2 and Al 3 could induce UDS and MCN in Vicia faba . There was a negative relationship between UDS and MCN, but it was not significant. The relationship between UDS and MCN was similar under the treatments of Cd 2 and Al 3 respectively. It was reliable using higher plant UDS test as the indicator to detect environmental mutagens within certain dosage range. |
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| Keywords: | Metal ion UDS MCN Correlation analysis Higher plant UDS test. |
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