Development and use of a vaccinia virus neutralization assay based on flow cytometric detection of green fluorescent protein |
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| Authors: | Earl Patricia L Americo Jeffrey L Moss Bernard |
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| Affiliation: | Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892-0445, USA. |
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| Abstract: | A rapid and sensitive neutralization assay is required to evaluate alternative smallpox vaccines. Here we describe the development and use of a 96-well plate, semi-automated, flow cytometric assay that uses a recombinant vaccinia virus expressing enhanced green fluorescent protein and which would be applicable to other viruses. |
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