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Enhancing functional expression of codon‐optimized heterologous enzymes in Escherichia coli BL21(DE3) by selective introduction of synonymous rare codons |
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| Authors: | Chao Zhong Ping Wei Yi‐Heng Percival Zhang |
| Affiliation: | 1. Department of Biological Systems Engineering, Virginia Tech, Blacksburg, Virginia;2. College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, China;3. +001‐540‐231‐7414001‐540‐231‐3199;4. Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China |
| Abstract: | |
| Keywords: | rare codon translation attenuation synonymous substitution functional protein expression directed evolution |