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Use of a protein engineering strategy to overcome limitations in the production of “Difficult to Express” recombinant proteins
Authors:Hirra Hussain  David I. Fisher  W. Mark Abbott  Robert G. Roth  Alan J. Dickson
Affiliation:1. 0161 306 4182 0000-0002-0918-4913 Manchester Institute of Biotechnology, Faculty of Science and Engineering, University of Manchester, Manchester, United Kingdom;2. AstraZeneca, Cambridge Science Park, Milton, Cambridge, United Kingdom;3. AstraZeneca, M?lndal, Sweden;4. Manchester Institute of Biotechnology, Faculty of Science and Engineering, University of Manchester, Manchester, United Kingdom
Abstract:
Keywords:recombinant protein production  difficult to express  mammalian expression system  tissue inhibitor of metalloproteinase  secretory pathway  protein engineering
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