Sequence arrangement in satellite DNA from the muskmelon |
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| Authors: | Bendich A J Taylor W C |
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| Affiliation: | Departments of Botany and Genetics, University of Washington, Seattle, Washington 98195. |
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| Abstract: | Two fractions of a satellite DNA from the muskmelon (Cucumis melo L.) isolated as a unimodal peak from CsCl gradients, differ in melting properties and complexity as estimated by reassociation kinetics. At 49.8 C, all of the low melting fraction was denatured and all of the high melting fraction was native. There were almost no partially denatured molecules detected in the electron microscope at this temperature. This observation provides direct evidence that the two fractions are not closely linked. We conclude that satellite I, the high tm, low complexity fraction, exists as a 600-nucleotide sequence in blocks of at least 67 tandem repeats. Since the complexity of the low melting fraction, satellite II, is greater than the size of the molecules in our assay, we can only say that the minimum size of each unit of satellite II is 2.5 × 107 daltons. It is unlikely that any spacer sequences are interspersed with either satellite. |
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