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Nucleic Acid and protein changes in relation to cold acclimation and freezing injury of korean boxwood leaves
Authors:Gusta L V  Weiser C J
Affiliation:Laboratory of Plant Hardiness, Department of Horticultural Science, University of Minnesota, St. Paul, Minnesota 55101.
Abstract:Quantitative and qualitative differences in nucleic acids of Korean boxwood (Buxus microphylla var. Koreana) leaves were determined by methylated albumin kieselguhr chromatography at different levels of cold hardiness. During cold acclimation there was an increase in RNA, mainly ribosomal RNA, with little or no change in DNA. The increase in ribosomal RNA was closely paralleled by an increase in water soluble and membrane bound proteins. As cold hardiness increased, ribonuclease activity declined.
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