A COMPARISON OF NUCLEAR DRY WEIGHTS DETERMINED BY CHEMICAL AND BY INTERFEROMETRIC METHODS |
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| Authors: | Arthur J. Hale and Ernest R. M. Kay |
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| Affiliation: | From the Institute of Physiology and the Department of Biochemistry, University of Glasgow, Scotland |
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| Abstract: | 1. The nuclei of cells from the thymus of the calf were isolated by three different techniques; the citric acid, the sucrose-calcium chloride, and the non-aqueous. 2. The mean dry weights of the nuclei were determined by chemical methods and by microscopic interferometry. There was a close correlation between the results from the interferometric and chemical methods. 3. The range of values about that mean was determined in each sample: the nuclei isolated in aqueous media contained approximately 45 per cent less material than those isolated in non-aqueous media. 4. The variations in dry weight with varying nuclear type are discussed. 5. The possible relationship between DNA content and dry weight is discussed. |
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