Epsilon-tubulin is an essential component of the centriole |
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| Authors: | Dutcher Susan K Morrissette Naomi S Preble Andrea M Rackley Craig Stanga John |
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| Affiliation: | Department of Genetics,Washington University School of Medicine, St Louis, Missouri 63110, USA. dutcher@genetics.wustle.edu |
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| Abstract: | Centrioles and basal bodies are cylinders composed of nine triplet microtubule blades that play essential roles in the centrosome and in flagellar assembly. Chlamydomonas cells with the bld2-1 mutation fail to assemble doublet and triplet microtubules and have defects in cleavage furrow placement and meiosis. Using positional cloning, we have walked 720 kb and identified a 13.2-kb fragment that contains epsilon-tubulin and rescues the Bld2 defects. The bld2-1 allele has a premature stop codon and intragenic revertants replace the stop codon with glutamine, glutamate, or lysine. Polyclonal antibodies to epsilon-tubulin show peripheral labeling of full-length basal bodies and centrioles. Thus, epsilon-tubulin is encoded by the BLD2 allele and epsilon-tubulin plays a role in basal body/centriole morphogenesis. |
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