Regulation of the shikimate pathway of carrot cells in suspension culture |
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| Authors: | Suzich J A Ranjeva R Hasegawa P M Herrmann K M |
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| Affiliation: | Department of Biochemistry, Purdue University, West Lafayette, Indiana 47907;Department of Horticulture, Purdue University, West Lafayette, Indiana 47907 |
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| Abstract: | The activity of the first enzyme of the shikimate pathway, 3-deoxy-d-arabino-heptulosonate 7-phosphate synthase, is demonstrated in extracts of Daucus carota cells grown in suspension culture. Maximum specific enzyme activity is found midway through the logarithmic growth of the culture; cells in lag and stationary phases of growth have lower enzyme levels. The enzyme is activated by tyrosine and tryptophan. The extent of activation varies during cell growth. |
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