|
|||||
|
|
Inside Cover: Optimized perfusion‐based CUBIC protocol for the efficient whole‐body clearing and imaging of rat organs (J. Biophotonics 5/2018) |
|
| Authors: | Paweł Matryba Lukasz Bozycki Monika Pawłowska Leszek Kaczmarek Marzena Stefaniuk |
| Affiliation: | 1. Laboratory of Neurobiology, Department of Molecular and Cellular Neurobiology, Nencki Institute of Experimental Biology, Warsaw, Poland;2. Department of Immunology, Medical University of Warsaw, Warsaw, Poland;3. Laboratory of Biochemistry of Lipids, Department of Biochemistry, Nencki Institute of Experimental Biology, Warsaw, Poland |
| Abstract: | Optical tissue clearing is a method allowing post‐mortem deep imaging of organs in three dimensions. By optimizing the CUBIC clearing protocol, the authors provide rapid and simple approach to clear the entire adult rat organism within as little as four days, which is accompanied by the variety of its staining and imaging techniques. The image was captured with polarizers and demonstrates transparent rodent heart with thread‐like crystals of clearing reagent. Further details can be found in the article by Pawe? Matryba et al. ( e201700248 ).  |
| Keywords: | light‐sheet microscope optical tissue clearing rat Thy‐1 GFP whole‐body clearing |